FlyBase Allele Report: Dmel\rept[GD4561]

General Information

Symbol

Dmel\rept^GD4561

Species

D. melanogaster

Name

FlyBase ID

FBal0210453

Feature type

allele

Associated gene

Dmel\rept

Associated Insertion(s)

Carried in Construct

P{GD4561}

Also Known As

REPTIN RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4561}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4561

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | spermatogenesis, with Scer\GAL4^VP16.bam

(Prozzillo et al., 2023)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

decreased size | adult stage, with Scer\GAL4^ey.PU

(Prozzillo et al., 2021)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

(Prozzillo et al., 2021)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^ey.PU

(Prozzillo et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of rept^GD4561 under the control of Scer\GAL4^Tub.PU from larval hatching onwards leads to defects in third instar salivary gland polytene chromosomes (smaller and with a thin and disorganized structure).

Expression under the control of Scer\GAL4^ey.PU leads to small eyes and microcephaly.

(Prozzillo et al., 2021)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the average size of neuroblasts in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing rept^GD4561 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development