Open Close
General Information
Symbol
Dmel\ryGD8894
Species
D. melanogaster
Name
FlyBase ID
FBal0210500
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

The fly stones in the ryGD8894 model are physically and visually similar to human kidney stones, though smaller in size.

Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ryGD8894 under the control of Scer\GAL4da.PU results in exuberant concretions in the malpighian tubule that have the appearance of small stones (known as 'fly stones'). There is an increase in the levels of xanthine and hypoxanthine and a decrease in uric acid in the whole fly. The stones themselves also contain xanthine and hypoxanthine, with hydroxyapatite as the primary calcium salt. The stones also contain high levels of zinc.

The number of fly stones formed when ryGD8894 under the control of Scer\GAL4da.PU is significantly reduced when flies are fed a low-yeast diet. Adding ZnSO[[4]] to the low-yeast food increases stone formation to the levels seen when the flies are raised on high yeast food. ZnSO[[4]] has no effect on the levels of stone formation in flies raised on high yeast food. Supplementing the food with N,N,N'N'-tetrakis- (2-pyridylmethyl)ethylenediamine (TPEN), a zinc-selective chelator, reduces stone formation in flies that have been fed a high-yeast diet, unless the food also contains supplemental Zn.

Expression of ryGD8894 under the control of Scer\GAL4da.PU results in a reduction in survival, with a median of lifespan of 3 days, compared with 60 days in controls.

Adults expressing ryGD8894 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in defects in locomotion.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of ZnT35CKK112697 suppresses the malpighian tubule concretions seen when ryGD8894 is expressed under the control of Scer\GAL4da.PU. The reduced lifespan is also partially suppressed.

Co-expression of ZnT35CKK112697 results in an increase in whole fly zinc levels compared to flies expressing ryGD8894 under the control of Scer\GAL4da.PU. The levels of other metals remain unchanged.

Expression of ZnT41FJF03053 suppresses the malpighian tubule concretions seen when ryGD8894 is expressed under the control of Scer\GAL4da.PU. The reduced lifespan is also partially suppressed.

Expression of ZnT63CKK102261 suppresses the malpighian tubule concretions seen when ryGD8894 is expressed under the control of Scer\GAL4da.PU. The reduced lifespan is also partially suppressed.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
ryGD8894
Name Synonyms
Secondary FlyBase IDs
    References (7)