FlyBase Allele Report: Dmel\ry[GD8894]

General Information

Symbol

Dmel\ry^GD8894

Species

D. melanogaster

Name

FlyBase ID

FBal0210500

Feature type

allele

Associated gene

Dmel\ry

Associated Insertion(s)

Carried in Construct

P{GD8894}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8894}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8894

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of xanthinuria

CEA

(Chi et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

The fly stones in the ry^GD8894 model are physically and visually similar to human kidney stones, though smaller in size.

(Chi et al., 2015)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

short lived, with Scer\GAL4^da.PU

(Chi et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Malpighian tubule, with Scer\GAL4^da.PU

(Chi et al., 2015)

Detailed Description

Statement

Reference

Expression of ry^GD8894 under the control of Scer\GAL4^da.PU results in exuberant concretions in the malpighian tubule that have the appearance of small stones (known as 'fly stones'). There is an increase in the levels of xanthine and hypoxanthine and a decrease in uric acid in the whole fly. The stones themselves also contain xanthine and hypoxanthine, with hydroxyapatite as the primary calcium salt. The stones also contain high levels of zinc.

The number of fly stones formed when ry^GD8894 under the control of Scer\GAL4^da.PU is significantly reduced when flies are fed a low-yeast diet. Adding ZnSO[[4]] to the low-yeast food increases stone formation to the levels seen when the flies are raised on high yeast food. ZnSO[[4]] has no effect on the levels of stone formation in flies raised on high yeast food. Supplementing the food with N,N,N'N'-tetrakis- (2-pyridylmethyl)ethylenediamine (TPEN), a zinc-selective chelator, reduces stone formation in flies that have been fed a high-yeast diet, unless the food also contains supplemental Zn.

Expression of ry^GD8894 under the control of Scer\GAL4^da.PU results in a reduction in survival, with a median of lifespan of 3 days, compared with 60 days in controls.

(Chi et al., 2015)

Adults expressing ry^GD8894 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in defects in locomotion.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8894

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ry^GD8894 has short lived phenotype, suppressible | partially by Scer\GAL4^da.PU/ZnT35C^KK112697

(Chi et al., 2015)

ry^GD8894 has short lived phenotype, suppressible | partially by ZnT41F^JF03053/Scer\GAL4^da.PU

(Chi et al., 2015)

ry^GD8894 has short lived phenotype, suppressible | partially by Scer\GAL4^da.PU/ZnT63C^KK102261

(Chi et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^da.PU, ry^GD8894 has Malpighian tubule phenotype, suppressible by Scer\GAL4^da.PU/ZnT35C^KK112697

(Chi et al., 2015)

Scer\GAL4^da.PU, ry^GD8894 has Malpighian tubule phenotype, suppressible by ZnT41F^JF03053/Scer\GAL4^da.PU

(Chi et al., 2015)

Scer\GAL4^da.PU, ry^GD8894 has Malpighian tubule phenotype, suppressible by Scer\GAL4^da.PU/ZnT63C^KK102261

(Chi et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ZnT35C^KK112697 suppresses the malpighian tubule concretions seen when ry^GD8894 is expressed under the control of Scer\GAL4^da.PU. The reduced lifespan is also partially suppressed.

Co-expression of ZnT35C^KK112697 results in an increase in whole fly zinc levels compared to flies expressing ry^GD8894 under the control of Scer\GAL4^da.PU. The levels of other metals remain unchanged.

Expression of ZnT41F^JF03053 suppresses the malpighian tubule concretions seen when ry^GD8894 is expressed under the control of Scer\GAL4^da.PU. The reduced lifespan is also partially suppressed.

Expression of ZnT63C^KK102261 suppresses the malpighian tubule concretions seen when ry^GD8894 is expressed under the control of Scer\GAL4^da.PU. The reduced lifespan is also partially suppressed.

(Chi et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v25172

w¹¹¹⁸; P{GD8894}v25172

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8894

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ry^GD8894

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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