FlyBase Allele Report: Dmel\slmb[GD10682]

General Information

Symbol

Dmel\slmb^GD10682

Species

D. melanogaster

Name

FlyBase ID

FBal0210615

Feature type

allele

Associated gene

Dmel\slmb

Associated Insertion(s)

Carried in Construct

P{GD10682}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10682}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10682

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Menkes disease

CEA

(Zhang et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Menkes disease

is ameliorated by cnc^KK101639

(Zhang et al., 2020)

is ameliorated by ATP7^GD3322

(Zhang et al., 2020)

is ameliorated by Ctr1A^{dN.UAS.Tag:FLAG}

(Zhang et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult abdomen, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

adult thorax, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^hs.PU

(Buster et al., 2013)

eye, with Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

larval brain | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

polytene chromosome, with Scer\GAL4^hs.PU

(Buster et al., 2013)

wing disc | third instar larval stage, with Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Detailed Description

Statement

Reference

Expression of slmb^GD10682 under the control of Scer\GAL4^pnr-MD237 leads to thoracic cleft and hypopigmentation on the abdomen and thorax when compared to control adults.

Expression of slmb^GD10682 under the control of Scer\GAL4^GMR.PFa leads to necrosis at the posterior margin of the eye when compared to control adults.

Expression of slmb^GD10682 under the control of Scer\GAL4^mex1.2.1 leads to an increase in survival on normal or CuCl2-supplemented food when compared to controls.

Expression of slmb^GD10682 under the control of Scer\GAL4^pnr-MD237 leads to increased cell death in third instar larval wing discs when compared to controls.

(Zhang et al., 2020)

Third instar larvae expressing slmb^GD10682 under the control of Scer\GAL4^hs.PU (in the presence of Dcr-2^Scer\UAS.cDa to increased RNAi) have completely unpaired salivary gland chromosomes.

(Buster et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

(Neumüller et al., 2011)

Adults expressing slmb^GD10682 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of slmb^GD10682 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD10682} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in adult/pharate lethality or undefined lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10682

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, slmb^GD10682 has visible | adult stage phenotype, non-enhanceable by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has visible | adult stage phenotype, non-enhanceable by Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has visible | adult stage phenotype, non-enhanceable by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, cnc^KK101639, slmb^GD10682 has visible phenotype, suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has visible | adult stage phenotype, suppressible | partially by ATP7^KK100396, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has visible | adult stage phenotype, suppressible by ATP7^KK100396, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has visible | adult stage phenotype, suppressible | partially by Ctr1A^{dN.UAS.Tag:FLAG}/Ctr1A^{dN.UAS.Tag:FLAG}/Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has visible | adult stage phenotype, suppressible | partially by cnc^KK101639/Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, slmb^GD10682 has visible | adult stage phenotype, non-suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has visible | adult stage phenotype, non-suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has visible | adult stage phenotype, non-suppressible by Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

NOT Enhancer of

Statement

Reference

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-enhancer of visible | adult stage phenotype of ATP7^KK100396, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-enhancer of visible | adult stage phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

NOT Suppressor of

Statement

Reference

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-suppressor of visible | adult stage phenotype of ATP7^KK100396, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-suppressor of visible | adult stage phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, non-enhanceable by Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, non-enhanceable by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, non-enhanceable by Scer\GAL4^pnr-MD237/cnc^KK101639

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has eye phenotype, non-enhanceable by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, cnc^KK101639, slmb^GD10682 has eye phenotype, suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, suppressible | partially by ATP7^KK100396, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has eye phenotype, suppressible by ATP7^KK100396, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has eye phenotype, suppressible | partially by Ctr1A^{dN.UAS.Tag:FLAG}/Ctr1A^{dN.UAS.Tag:FLAG}/Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has eye phenotype, suppressible | partially by cnc^KK101639/Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

NOT suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, non-suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, non-suppressible by Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Scer\GAL4^pnr-MD237, slmb^GD10682 has adult thorax phenotype, non-suppressible by Scer\GAL4^pnr-MD237/cnc^KK101639

(Zhang et al., 2020)

Scer\GAL4^GMR.PFa, slmb^GD10682 has eye phenotype, non-suppressible by Ctr1A^{dN.UAS.Tag:FLAG}, Scer\GAL4^GMR.PFa

(Zhang et al., 2020)

NOT Enhancer of

Statement

Reference

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-enhancer of adult thorax phenotype of ATP7^KK100396, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-enhancer of adult thorax phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

NOT Suppressor of

Statement

Reference

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of ATP7^KK100396, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

slmb^GD10682, Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Zhang et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Thoracic cleft but not hypopigmentation on thorax phenotype resulted from the expression of slmb^GD10682 (under the control of Scer\GAL4^pnr-MD237) is suppressible by expression of ATP7^KK100396 (under the control of Scer\GAL4^pnr-MD237).

(Zhang et al., 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v34273