FlyBase Allele Report: Dmel\sls[GD9680]

General Information

Symbol

Dmel\sls^GD9680

Species

D. melanogaster

Name

FlyBase ID

FBal0210622

Feature type

allele

Associated gene

Dmel\sls

Associated Insertion(s)

Carried in Construct

P{GD9680}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9680}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9680

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

lethal, with Scer\GAL4^Mef2.PR

(Dietzl et al., 2007)

lethal - all die before end of larval stage, with Scer\GAL4^2-286

(Nakaoka et al., 2017)

lethal - all die before end of larval stage, with Scer\GAL4^phtm.PU

(Nakaoka et al., 2017)

lethal - all die during embryonic stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere | adult stage, with Scer\GAL4^Hk-UH3

(Katti et al., 2017)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Adult flies expressing sls^GD9680 under the control of Scer\GAL4^Hk-UH3 show no gross morphological defects in indirect flight muscles with largely normal sarcomere organization, but some tearing of the sarcomere is occasionally observed.

(Katti et al., 2017)

Expression of sls^GD9680 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4^phm.PU or Scer\GAL4^2-286 is second/third larval stage lethal.

(Nakaoka et al., 2017)

Adults expressing sls^GD9680 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in embryonic lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with discontinuous Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0%, 20-30% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30%, 70-80%, 80-90% or 100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development