FlyBase Allele Report: Dmel\tws[GD10742]

General Information

Symbol

Dmel\tws^GD10742

Species

D. melanogaster

Name

FlyBase ID

FBal0210829

Feature type

allele

Associated gene

Dmel\tws

Associated Insertion(s)

Carried in Construct

P{GD10742}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10742}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10742

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing tws^GD10742 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in duplication of bristle hairs on the notum in 30-40% or 60-70% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10742

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

tws^GD10742, Scer\GAL4^en.PU is a suppressor | partially of abnormal cell death | third instar larval stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^en.PU

(Pechkovsky et al., 2013)

tws^GD10742, Scer\GAL4^GMR.PF is a suppressor | partially of visible | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

tws^GD10742, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal size | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

tws^GD10742, Scer\GAL4^en.PU is a suppressor | partially of wing disc phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^en.PU

(Pechkovsky et al., 2013)

tws^GD10742, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of tws^GD10742 partially suppresses the wing disc phenotype seen when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^en.PU at 29[o]C. Only 34% of wing discs contain a cluster of dead cells, compared to 100% when Zzzz\E4orf4^Scer\UAS.cPa is expressed alone.

Expression of tws^GD10742 partially suppresses the eye phenotypes seen in males when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^GMR.PF at 29[o]C. Eye size is restored to 79% of controls.

(Pechkovsky et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v34340

w¹¹¹⁸; P{GD10742}v34340

Notes on Origin

Discoverer