FlyBase Allele Report: Dmel\vkg[GD5246]

General Information

Symbol

Dmel\vkg^GD5246

Species

D. melanogaster

Name

FlyBase ID

FBal0210863

Feature type

allele

Associated gene

Dmel\vkg

Associated Insertion(s)

Carried in Construct

P{GD5246}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5246}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5246

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing vkg^GD5246 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5246

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Avic\GFP^RNAi.R.UAS, Scer\GAL4^Cg.PA, Scer\GAL80^ts.αTub84B, vkg^G00454, vkg^GD5246 has lethal - all die before end of pupal stage | heat sensitive phenotype

(Pastor-Pareja and Xu, 2011)

Phenotype Manifest In

Other

Statement

Reference

Avic\GFP^RNAi.R.UAS, Scer\GAL4^Cg.PA, Scer\GAL80^ts.αTub84B, vkg^G00454, vkg^GD5246 has imaginal disc | heat sensitive phenotype

(Pastor-Pareja and Xu, 2011)

Avic\GFP^RNAi.R.UAS, Scer\GAL4^Cg.PA, Scer\GAL80^ts.αTub84B, vkg^G00454, vkg^GD5246 has larval ventral nerve cord | heat sensitive phenotype

(Pastor-Pareja and Xu, 2011)

Avic\GFP^RNAi.R.UAS, Scer\GAL4^Cg.PA, Scer\GAL80^ts.αTub84B, vkg^G00454, vkg^GD5246 has embryonic/larval salivary gland | heat sensitive phenotype

(Pastor-Pareja and Xu, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

At a permissive temperature (18[o]C) expression of vkg^G454 and vkg^GD5246 under the control of Scer\GAL4^Cg.PA and Scer\GAL80^ts.αTub84B knocks down expression of vkg. These flies develop into normal adults, although pupal lethality results when the larvae are transferred to restrictive temperature after completion of embryogenesis. Dissection of these larvae 96 hours after the temperature shift reveals highly aberrant shapes in several organs. Imaginal discs, consisting of highly columnar epithelial cells, appear flattened while the ventral nerve cord portion of the central nervous system is strikingly elongated, reminiscent of embryonic defects in ventral nerve cord condensation. Additionally, the ducts and imaginal rings of the salivary glands are dilated, resulting in an expanded lumen.

(Pastor-Pareja and Xu, 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v16986

w¹¹¹⁸; P{GD5246}v16986

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5246

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

vkg^GD5246

vkg^RNAi

(Csordás et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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