FlyBase Allele Report: Dmel\Or67d[GAL4-1]

General Information

Symbol

Dmel\Or67d^GAL4-1

Species

D. melanogaster

Name

FlyBase ID

FBal0210948

Feature type

allele

Associated gene

Dmel\Or67d

Associated Insertion(s)

TI{GAL4}Or67d^GAL4-1

Carried in Construct

Also Known As

Or67d^GAL4, Or67d-Gal4

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Kurtovic et al., 2007, Kurtovic et al., 2007)

I-SceI

(Kurtovic et al., 2007)

FLPase

(Kurtovic et al., 2007)

I-CreI

(Kurtovic et al., 2007)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}Or67d^GAL4-1

Cytology

Description

The Or67d open reading frame has been replaced by sequence encoding a GAL4 driver flanked on each side by loxP sites.

(Kurtovic et al., 2007)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult olfactory receptor neuron Or67d

(Li et al., 2013)

Additional Information

Statement

Reference

Marker for

adult olfactory receptor neuron Or67d

(Li et al., 2013)

Reflects expression of

Or67d

(Li et al., 2013)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior

(Kurtovic et al., 2007, Kurtovic et al., 2007)

abnormal courtship behavior | male

(Keleman et al., 2012)

abnormal neurophysiology

(Kurtovic et al., 2007)

Phenotype Manifest In

trichoid sensillum of antennal segment 3

(Kurtovic et al., 2007)

Detailed Description

Statement

Reference

As in wild type, Or67d^GAL4-1 mutant males exhibit inhibition of female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.

(Yuan et al., 2014)

Naive mutant males court virgin and mated males equally and do not benefit from prior training.

(Keleman et al., 2012)

Or67d^GAL4-1 males show relatively wild-type levels of aggression to male flies in which the hydrocarbon-producing oenocytes have been eliminated (through expression of W^Scer\UAS.cZa, and Avic\GFP^{Stinger.Scer\UAS.T:nls-tra} under the control of Scer\GAL4^desat1.PB and Scer\GAL80^ts.αTub84B at 18[o]C during development and 25[o]C at the adult stage).

(Wang et al., 2011)

The courtship index of Or67d^GAL4-1 males paired with wildtype females is not significantly different from wild-type controls. Addition of 11-cis-vaccenyl acetate (cVa) to the mating chamber fails to suppress courtship in mutant males towards wild-type females. Indeed, the courtship index of these males in the presence of cVa is significantly higher than in wild-type male controls.

(Ronderos and Smith, 2010)

Synthetic 11-cis-vaccenyl acetate (cVA) does not promote male-male aggression in Or67d^GAL4-1 males, in contrast to wild-type males.

(Wang and Anderson, 2010)

Or67d^GAL4-1 males paired with wild-type males show a significantly higher male-male courtship index than that of control males paired with wild-type males.

Or67d^GAL4-1 females mated to wild-type males show a higher copulation latency than control females mated to wild-type males.

(Kurtovic et al., 2007)

The male pheromone cVA ([Z]-11-octadecenyl acetate) elicits a rapid and robust firing response in the T1 trichoid sensilla of control Or67d⁺¹ flies but not in those of Or67d^GAL4-1 flies. Male and female Or67d^GAL4-1 flies do not produce a detectable electroretinogram response to cVA (in contrast to controls), but they show a normal electroretinogram response to ethanol.

When paired with virgin females, Or67d^GAL4-1 males court at levels comparable to those of control males. However, when Or67d^GAL4-1 males are paired with wild-type males, they show a roughly threefold higher courtship activity than control males paired with wild-type males.

Or67d^GAL4-1 females mated to wild-type males show a higher latency to copulation than control females mated to wild-type males.

Virgin Or67d^GAL4-1 females are courted as vigorously as control females when paired with wild-type males.

Application of the male pheromone cVA ([Z]-11-octadecenyl acetate) to the abdomens of virgin females suppresses courtship by control males but not by Or67d^GAL4-1 males in a single-pair courtship assay.

(Kurtovic et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Or67d^GAL4-1 is a suppressor of abnormal courtship behavior | heat sensitive phenotype of Scer\GAL4^ple.PF, TrpA1^UAS.(B).cKa

(Keleman et al., 2012)

Or67d^GAL4-1 is a suppressor of abnormal neurophysiology phenotype of lush^t.D118A

(Ronderos and Smith, 2010)

Or67d^GAL4-1 is a suppressor of abnormal courtship behavior phenotype of lush^t.D118A

(Ronderos and Smith, 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The suppression of courtship towards mated females which is seen in males expressing TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^ple.PF which have been shifted to 30[o]C for 45 minutes and subsequently returned to 25[o]C is not seen in a Or67d^GAL4-1 background.

(Keleman et al., 2012)

The lush^t.D118A-induced activation of T1 neurons is mediated by Or67d as an Or67d^GAL4-1 background abolishes this activating effect.

A Or67d^GAL4-1 background completely reverses the lush^t.D118A-induced suppression of male courtship, indicating these behavioral changes are mediated through T1 neurons.

A Or67d^GAL4-1 background completely suppresses the increase in courtship behaviour seen in lush^t.D118A females.

(Ronderos and Smith, 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Or67d^GAL4-1 is rescued by Scer\GAL4^Or67d-GAL4-1/Or67d^UAS.cKa

(Kurtovic et al., 2007)

Or67d^GAL4-1 is rescued by Scer\GAL4^Or67d-GAL4-1/Or67d^UAS.cKa

(Kurtovic et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

604607

w^*; P{UAS-mCD8::GFP.L}LL5; TI{GAL4}Or67d^GAL4-1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

OR67d^GAL4

(Syed et al., 2010)

Or67d^-

(Yuan et al., 2014)

Or67d^GAL4-1

Or67d^GAL4

(Wu et al., 2022, Li et al., 2016, Wang et al., 2016, Gomez-Diaz et al., 2013, Li et al., 2013, Keleman et al., 2012, Wang and Anderson, 2010, Kurtovic et al., 2007, Kurtovic et al., 2007, Kyriacou, 2007)

Or67d^GAL4[1]

(Kurtovic et al., 2007, Kurtovic et al., 2007)

Or67d^Gal4

(Khallaf et al., 2020, Mercier et al., 2018, Kohl et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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