Germaria that consist mostly of TSG101² cells show misalignment of cysts and increased cell numbers between egg chambers.

TSG101² mutant oocytes rarely contain intralumenal vesicles and isolated multivesicular bodies. They also exhibit irregularly shaped yolk granules.

TSG101² mosaic eyes exhibit N-dependent overgrowth and give rise to bulging adult eyes.

Tissue in mutant eye discs (generated using the eyFLP-cell lethal system) loses apicobasal polarity and fails to undergo terminal differentiation. The mutant eye discs show overgrowth compared to wild type.

Larvae containing mutant eye discs generated using the eyFLP-cell lethal system are increased in size compared to control larvae when examined as pupariation commences. The animals do not develop into adults.

Eyes and heads of adult flies mosaic for TSG101² are dramatically enlarged and misshapen, despite being composed largely of wild-type twin-spot cells. Adult retinal sections show disorganized cellular architecture in these eye. Many ommatidia have missing or extra photoreceptors and there is evidence of fusion events between adjacent clusters. Rare TSG101² mutant cells appear as bloated cell bodies with lightly staining rhabdomeres. The the late third instar eye-antennal discs of these animals are also enlarged, again despite being composed largely of wild-type twin-spot cells. TSG101² mutant cells in these discs are more rounded than adjacent wild-type cells and marker analysis indicates some disruption of apical-basal polarity. The patterns of BrdU incorporation in TSG101² mosaic eye discs are disorganized and the number of BrdU-labeled nuclei increases in proximity to TSG101² mutant cells in the eye and antennal discs.

When RpL14⁺ TSG101² homozygous somatic clones are induced in a RpL14¹/+ TSG101² background, these clones grow to almost completely fill the discs. The resulting animals reach the wandering third instar larval stage 4 days later than control larvae, and, when they do, they are enlarged. A small fraction of these animals pupate and die before becoming pharate adults. At control larval stage L3, the discs of these animals are mispatterned but not enlarged. However, in larvae surviving past control pupariation stage, these clone cells continue to grow, producing large masses that lack normal disc morphology. These masses are composed of folded and convoluted sheets of cells fused together and they often include a distended sac-like structure.

TSG101² has eye | somatic clone phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

TSG101² has ommatidium | somatic clone | cell non-autonomous phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶