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General Information
Symbol
Dmel\Dscam14.3-6.36-9.25-17.1
Species
D. melanogaster
Name
FlyBase ID
FBal0213029
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 4.5kb Dscam promoter fragment from -261 to +4309 bp (this fragment includes the 5' untranslated sequences of exon 1 and up to the exon 2 ATG codon) is fused to a Dscam cDNA that encodes exons 2-18 followed by a genomic fragment encoding Dscam exons 18-24. The cDNA fragment contains the alternative exons 4.3, 6.36, 9.25 and 17.1.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies carrying Dscam4.3-6.36-9.25-17.1 in a wild-type background show no defects in the morphogenesis of mushroom body α/β neurons.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The self-avoidance defects (dendrite crossings) seen in Dscam21/Dscam23 class I da neurons are rescued by expression of Dscam4.3-6.36-9.25-17.1.

Dscam4.3-6.36-9.25-17.1 fully rescues the projection neuron dendrite defects, but has no effect on the reduced number of calyx boutons seen in Dscam18 clones.

Dscam4.3-6.36-9.25-17.1 shows only minimal rescue of the axon branching defects seen in single cell clones of mushroom body α/β neurons that are homozygous for Dscam18; 28% of clones show lack of segregation of sister branches and 34% show generation of additional branches at the bifurcation points (compared to 35% and 42% respectively in the absence of Dscam4.3-6.36-9.25-17.1).

Dscam4.3-6.36-9.25-17.1 fails to rescue the early larval lethality of Dscam18/DscamB17-1 animals.

Co-expression of both Dscam4.3-6.36-9.25-17.1 and Dscam4.3-6.36-9.25-17.2 partially rescues the early larval lethality of Dscam18/DscamB17-1 animals, such that some some animals survive to the adult stage. These adults are vegetative after eclosion and need special care for survival (some of these flies can survive for 3 days within wet chambers on grape juice agar plates). The rescued adults show multiple signs of poor brain development; all major brain compartments (including the supraesophageal ganglion, the subesophageal ganglion and the optic lobes) appear rudimentary and the paired brain lobes are incompletely fused. Loss of dorsal or medial mushroom body lobes is seen and the brains lack ellipsoid body-like structures.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Dscam14.3-6.36-9.25-17.1
Dscam4.3-6.36-9.25-17.1
Name Synonyms
Secondary FlyBase IDs
    References (3)