FlyBase Allele Report: Dmel\Dscam1[10.27.25]

General Information

Symbol

Dmel\Dscam1^10.27.25

Species

D. melanogaster

Name

FlyBase ID

FBal0215825

Feature type

allele

Associated gene

Dmel\Dscam1

Associated Insertion(s)

TI{TI}Dscam1^10.27.25

Carried in Construct

Also Known As

Dscam^10.27.25

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Hattori et al., 2007)

FLPase

(Hattori et al., 2007)

gene targeting by homologous recombination

(Hattori et al., 2007)

Progenitor genotype

Dscam1^ED69

(Hattori et al., 2007)

Dscam1^{5'HR-10.27.25}

(Hattori et al., 2007)

Associated Insertion(s)

TI{TI}Dscam1^10.27.25

Cytology

Description

Recombination between the single FRT sites that are present on each of Dscam1^ED69 and Dscam1^{5'HR-10.27.25} has resulted a single copy of Dscam1 in which the genomic region encoding exons 3 to 16 has been replaced with cDNA sequences. This copy of Dscam1 encodes a single ectodomain isoform, containing the variable exons 4.10, 6.27 and 9.25. A single FRT site is present following the cDNA sequences.

(Hattori et al., 2007)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Kim et al., 2013)

abnormal neuroanatomy | dominant

(Hattori et al., 2007)

abnormal neuroanatomy | embryonic stage

(Hattori et al., 2007)

abnormal neuroanatomy | embryonic stage (with Dscam1^3.31.8)

(Hattori et al., 2007)

abnormal neuroanatomy | embryonic stage (with Dscam1^6.5.9)

(Hattori et al., 2007)

abnormal neuroanatomy | embryonic stage (with Dscam1²³)

(Hattori et al., 2007)

abnormal neuroanatomy | pupal stage

(Hattori et al., 2007)

lethal - all die before end of larval stage (with Dscam1^3.31.8)

(Hattori et al., 2007)

lethal - all die before end of larval stage (with Dscam1^6.5.9)

(Hattori et al., 2007)

lethal - all die before end of larval stage | recessive

(Hattori et al., 2007)

some die during larval stage (with Dscam1^3.31.8)

(Hattori et al., 2007)

some die during larval stage (with Dscam1^6.5.9)

(Hattori et al., 2007)

some die during larval stage | recessive

(Hattori et al., 2007)

Phenotype Manifest In

antennal lobe

(Hattori et al., 2007)

antennal lobe glomerulus

(Hattori et al., 2007)

larval dorsal multidendritic neuron ddaC

(Kim et al., 2013)

larval longitudinal connective

(Hattori et al., 2007)

larval longitudinal connective (with Dscam1^3.31.8)

(Hattori et al., 2007)

larval longitudinal connective (with Dscam1^6.5.9)

(Hattori et al., 2007)

larval longitudinal connective (with Dscam1²³)

(Hattori et al., 2007)

lobe system of adult mushroom body

(Hattori et al., 2007)

olfactory receptor neuron

(Hattori et al., 2007)

presumptive embryonic/larval central nervous system

(Hattori et al., 2007)

presumptive embryonic/larval central nervous system (with Dscam1^3.31.8)

(Hattori et al., 2007)

presumptive embryonic/larval central nervous system (with Dscam1^6.5.9)

(Hattori et al., 2007)

presumptive embryonic/larval central nervous system (with Dscam1²³)

(Hattori et al., 2007)

synapse

(Kim et al., 2013)

Detailed Description

Statement

Reference

C4 da neurons expressing the single Dscam1 isoform containing exons 4.10, 6.27, and 9.25 (Dscam1^10.27.25) exhibit defective targeting of the synaptic terminals. 47% of Dscam1^10.27.25 ddaC neurons completely lose their anterior branches and 29.4% lose their contralateral branches, while 100% of wild-type controls exhibit both branches.

(Kim et al., 2013)

Homozygotes, Dscam^10.27.25/Dscam^3.31.8 and Dscam^10.27.25/Dscam^6.5.9 animals die in early larval development.

Over 75% of Dscam^10.27.25/Dscam²³ animals survive to late pupal stages.

Homozygous, Dscam^10.27.25/Dscam^3.31.8 and Dscam^10.27.25/Dscam^6.5.9 embryos show defects in the organisation of the central nervous system, showing severely disrupted longitudinal tracts and aberrant midline crossing.

Dscam^10.27.25/Dscam²³ embryos show defects in the organisation of the central nervous system, showing disrupted longitudinal tracts.

Dscam^10.27.25/Dscam^- animals have a highly disorganised antennal lobe with no distinct glomerular structure and olfactory receptor neurons form many ectopic termini throughout the antennal lobe.

Almost all Dscam^10.27.25/Dscam^- mushroom bodies completely lack one lobe (typically the dorsal lobe is missing). Dscam^3.31.8/+ animals also show defects in the mushroom body in approximately 90% of cases; either one mushroom body lobe is absent (approximately 60% of cases) or one mushroom body lobe is thinner than the other (approximately 30% of cases).

Sister branches of single Dscam^10.27.25 mushroom body neurons in a Dscam^FRT control background (generated using intragenic MARCM) segregate into the two mushroom body lobes with high fidelity.

(Hattori et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dscam1^10.27.25

(Kim et al., 2013)

Dscam^10.27.25

(Hattori et al., 2007)

dscam^10.27.25

(Liu et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close