FlyBase Allele Report: Dmel\Dnai2[MB06262]

General Information

Symbol

Dmel\Dnai2^MB06262

Species

D. melanogaster

Name

FlyBase ID

FBal0217857

Feature type

allele

Associated gene

Dmel\Dnai2

Associated Insertion(s)

Mi{ET1}Dnai2^MB06262

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Karak et al., 2015)

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}Dnai2^MB06262

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:11,698,644..11,698,644 [-]

(Karak et al., 2015, Bellen, 2008.8.29)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of sensorineural hearing loss

CEA

(Senthilan et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception

(Karak et al., 2015)

abnormal auditory perception | adult stage

(Senthilan et al., 2012)

abnormal neurophysiology | adult stage

(Senthilan et al., 2012)

male fertile

(Karak et al., 2015)

Phenotype Manifest In

Johnston organ

(Karak et al., 2015)

spermatozoon

(Karak et al., 2015)

Detailed Description

Statement

Reference

Electrical sound responses of Johnston's organ neurons are virtually abolished in Dnai2^MB06262 mutant flies, although some residual responses to intense sound stimuli are retained. Mechanical amplification is also completely abolished.

No gross morphological defects are seen in the Johnston's organ neurons in Dnai2^MB06262 mutant flies but the outer dynein arms in the proximal region of the cilium are selectively lost.

Homozygous Dnah3^MB05004 mutant males are fertile and sperm appear motile. There is a significant reduction in sperm competition compared with controls.

(Karak et al., 2015)

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification.

(Senthilan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Dnai2^MB06262 has abnormal auditory perception phenotype, non-suppressible by iav^K1

(Karak et al., 2015)

NOT Suppressor of

Statement

Reference

Dnai2^MB06262 is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Dnai2^MB06262 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

As in either mutant alone, iav¹ Dnai2^MB06262 double mutants entirely lack sound-evoked electrical potentials. The excessive mechanical amplification seen in single iav¹ mutants is entirely abolished in the double mutants, as in single Dnai2^MB06262 mutants.

(Karak et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dnai2^MB06262 is rescued by Scer\GAL4^nan.PK/Dnai2^UAS.YFP

(Karak et al., 2015)

Dnai2^MB06262 is rescued by Dnai2^+t20

(Karak et al., 2015)

Comments

Expression of Dnai2^MB06262 rescues the sound-evoked electrical and mechanical response defects seen in Dnai2^+t20 mutant flies.

Expression of Dnai2^{Scer\UAS.T:Avic\GFP-YFP} in chordotonal neurons under the control of Scer\GAL4^nan.PK rescues the sound-evoked electrical and mechanical response defects seen in Dnai2^MB06262 mutant flies.

(Karak et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25491

w¹¹¹⁸; Mi{ET1}Dnai2^MB06262

Notes on Origin

Discoverer

Comments

Precise excision of the Mi{ET1}Dnai2^MB06262 element restores the sound-evoked electrical and mechanical response defects seen in Dnai2^MB06262 mutant flies. The loss of the outer dynein arms in the ciliary axonemes of Johnston's organ neurons is also restored.

(Karak et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG6053^MB06262

(Senthilan et al., 2012, Bellen, 2008.8.29)

Dmdnai2¹

(Karak et al., 2015)

Dnai2^MB06262

Dnai2MB06262

(Jiao et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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