Amino acid replacement: Q455term.
Nucleotide substitution: C1363T.
C6815795T
C1363T
Q455term | iav-PA
Q455term
iavK1 mutant adult females have no difference in preference for softer plain, or 100 mM sucrose-containing, agarose for egg laying, compared to controls.
iavK1 mutant adults have no difference in spiking or mechanotransduction currents of mechanosensory neurons of the labellar taste bristles in response to mechanical stimuli, compared to controls; there is also no effect on spiking of mechanosensory neurons of labellar taste pegs, compared to controls.
iavK1 mutant adults do not show any defects in sensing food hardness and like the wild-type flies prefer softer food with lower sugar content (0.5mM sucrose, 0.2% agarose) to a hard food with higher sugar content (1mM sucrose, 2% agarose) in a binary choice assay.
iavK1 mutant larvae do not have alterations in defecation rhythm compared to controls.
Unlike wild-type, mutant flies show no sensitization to a second exposure to cocaine when assayed by behavioural scoring; the behavioural responses to an initial and a second exposure to cocaine were indistinguishable. Feeding the mutant flies with tyramine rescues this phenotype. Wild-type flies fed tyramine become very sick and often die after exposure to cocaine whereas mutant flies remain healthy for several days under these conditions.
Intermediate experience-dependent courtship modification (EDCM) phenotype.
Lifespan of males and females is reduced by 6 days. Males have a slightly shorter lifespan, females outlive males by approximately 12 days. For each age mutant flies are more attractive than wild type flies, the attractiveness does decrease with age. Males and females have normal eclosion rhythm. Mutants are susceptible to p-Cresol.
In tests of homozygous females (with normal males), mating propensity reduced and courtship durations extended, though such females showed normal compositions of cuticular hydrocarbons and were highly attractive to courting males. Mutant males with normal females exhibited slightly reduced mating success; mutant males crossed with mutant females had very low mating success rate.
Open-field activity tests of mutant adults showed reduced speed as well as amount of locomotion (the former meaning number of squares in the activity chambered visited/unit time, the latter the proportion of time spent moving); male activity levels higher than for females (also found for wild-type); lower than wild-type levels of activity at all ages between time of eclosion and approximately one-month; yet, unlike wild type, speed and amount of activity increase in days 1 and 2 after eclosion (before reaching a much lower than normal plateau).
Mutant adults are extremely inactive, with normal external morphology; population of mutants remains quiet and spread out evenly in a container; will walk or fly when container disturbed, but settles into inactivity soon afterward.
iavK1 has abnormal auditory perception phenotype, suppressible by Dnah3MB05004
iavK1 is a non-suppressor of abnormal auditory perception phenotype of Dnai2MB06262
As in either mutant alone, iav1 Dnai2MB06262 double mutants entirely lack sound-evoked electrical potentials. The excessive mechanical amplification seen in single iav1 mutants is entirely abolished in the double mutants, as in single Dnai2MB06262 mutants.
iavK1 is rescued by iavCterm.EGFP
iavK1 is not rescued by iavNterm.EGFP
Kaplan, 1977.