Imprecise excision of the P{EPgy2}mus309EY03745 insertion, resulting in a deletion that removes mus309 sequences. The deletion is unusual in that it begins downstream of the site of the original P{EPgy2}mus309EY03745 insertion. It may have arisen after an initial transposition of the P{EPgy2} element followed by deletion. The deletion begins after the start codon, but results in a frameshift.
Embryos derived from homozygous females have a reduced hatch rate compared to those derived from wild-type females.
Only 76% of the embryos derived from homozygous females have cellularised by 4-6 hours after egg laying, and only 59% have gastrulated by this time (in contrast to embryos derived from wild-type females where 99% have cellularised and 96% have gastrulated by this time).
Homozygous and mus309N2/mus309D2 females show an increased rate of X chromosome meiotic nondisjunction compared to wild-type females.
Homozygous females show a changed crossover frequency on chromosome arm 2L compared to wild-type females. There is a 66% decrease in crossover frequency in two central intervals (dp to wg and wg to b), but in the interval spanning the centric heterochromatin (pr to cn) the crossover frequency is increased 2.5 fold in the mutant females.
Homozygous larvae are hypersensitive to gamma radiation, showing reduced survival to adulthood after irradiation with doses of radiation that do not have a large effect on the survival of wild-type larvae.
Studies of excision and repair events of P{hswa} in homozygous, mus309N2/mus309D2 and mus309N2/mus309D3 males indicate that synthesis-dependent strand annealing (SDSA) is severely compromised in these animals.
mus309N2/mus309D2 males show elevated rates of mitotic crossing over compared to control males.
BlmN2/BlmD2, Genunspecified/Df(3L)Exel6103 has lethal | pharate adult stage phenotype
BlmN2/BlmD2, mus312D1/mus312Z1973 has lethal | pupal stage phenotype
BlmN2/BlmD2, Genunspecified/Df(3L)Exel6103 has imaginal disc | larval stage phenotype
BlmN2/BlmD2, Genunspecified/Df(3L)Exel6103 has condensed chromosome phenotype
Mitotic neuroblasts in Genunspecified/Df(3L)Exel6103 mus309D2/mus309N2 double mutant larvae show an increased frequency of broken chromosomes compared to wild type.
mus309D2/mus309N2 mus312D1/mus312Z1973 double mutant larvae generally lack imaginal discs (although small, severely underdeveloped discs are sometimes seen in third instar larvae).
