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FB2026_01
,
released March 12, 2026
A 9bp deletion in mt:ND2 (atattaaga) destroys the BglII restriction enzyme site.
Mutants display bang sensitivity that progressively worsens with age - females by 10 days, males by 26 days of age.
Median lifespan is reduced compared to wild type, with female survival being shorter than male.
Female mutants are fully paralyzed following exposure to 39[o]C for 6 minutes and require prolonged recovery after returning to room temperature, whereas controls are not paralysed under these conditions.
Young female mutants display significantly reduced flying ability compared to controls.
Young and middle-aged female mutants take longer to recover from exposure to hypercarbic and hypoxic conditions than controls. Young female mutants housed in 100% oxygen have a significantly shorter lifespan compared to controls.
No abnormalities are seen in the flight muscles of middle-aged or old female mutants, or in the brains of middle-aged female mutants. However, neurodegenerative vacuoles in the brains of old female mutants are significantly larger and more numerous than in controls.
Female mutants do not show a significant alteration in the formation of reactive oxygen species, compared to controls.
Homoplasmic mt:ND2del1 mutant flies exhibit a climbing defect
mt:CoIT300I, mt:ND2del1 has lethal | temperature conditional phenotype
Bsub\BglIIUASp.Tag:Mito(Cs1), Scer\GAL4nanos.PU, mt:CoIT300I, mt:ND2del1 has female semi-sterile | maternal effect | temperature conditional phenotype
mt:CoIR301Q, mt:ND2del1 has male sterile phenotype
Bsub\BglIIUASp.Tag:Mito(Cs1), Scer\GAL4nanos.PU, mt:CoIR301Q, mt:ND2del1 has female semi-sterile | maternal effect | temperature conditional phenotype
Flies heteroplasmic for mt:CoIT300I and mt:ND2del1 are healthy at 29[o], even when the temperature-sensitive mt:CoIT300I mutant is in high abundance.
Simultaneous expression of Bsub\BglIIScer\UAS.P\T.T:Mito-kdn and Xvas\XhoIScer\UAS.P\T.T:Mito-kdn,T:Hsap\MYC restriction enzymes under the control of Scer\GAL4nos.PU in flies that are homoplasmic for mt:ND2del1 (which lacks the BglII restriction site) lead to sterility in females. Upon selection, about 1% percent of the females become weakly fertile producing a small number of escaper progeny that harbor a variety of mutations inactivating the XhoI restriction site. In contrast, when the flies are heteroplasmic for mt:CoIR301Q and mt:ND2del1 (in a 50:50 ratio), the Scer\GAL4nos.PU-driven expression of both Bsub\BglIIScer\UAS.P\T.T:Mito-kdn and Xvas\XhoIScer\UAS.P\T.T:Mito-kdn,T:Hsap\MYC does not lead to sterility - more than 90% of the females are fertile and most display fecundity comparable to that of wild-type females. Scer\GAL4ey.PU-driven expression of the two restriction enzymes at 29[o]C results in pupal lethality (headless pupae) in a vast majority of flies and the few escapers are eyeless or have a small eye phenotype.
90% of Drosophila melanogaster heteroplasmic females carrying both a D. yakuba mitochondrial genome harboring the Dyak\mt:ND1R274W mutation (which removes the XhoI site) and the D. melanogaster mt:ND2del1 mutant genome and expressing Bsub\BglIIScer\UAS.P\T.T:Mito-kdn and Xvas\XhoIScer\UAS.P\T.T:Mito-kdn,T:Hsap\MYC under the control of Scer\GAL4nos.PU are sterile, the F1 progeny from the minority of fertile females carry recombinant Dmel/Dyak mt genomes in which both the BglII and XhoI restriction sites are mutated.