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General Information
Symbol
Dmel\Atg13UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0221505
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Atg13-GFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Atg13 which is tagged with EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Clones of cells in the fat body expressing Atg13Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Act5C.PP fail to induce autophagy in response to starvation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU at 18[o]C results in reduced viability and fertility, as well as eye degeneration. Raising the flies at 18[o]C restores fertility, whilst maintaining the eye degeneration. Retinal cells such as photoreceptors and cone cells exhibit degeneration and there is complete disarray of the ommatidia structure and accumulation of membrane-dense vacuoles. After 4 weeks the retinal structure has completely degenerated except for the corneal lens exocuticle. Prominent cell death is seen in the differentiation ommatidia in eye disc and autophagic flux is increased.

Expression of GyfHM05106 dramatically suppresses the eye degeneration and eye disc cell death seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C. However, the photoreceptor morphology is not fully restored. The increased autophagic flux in eye discs is also suppressed.

Expression of Atg1JF02273 completely suppresses the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

Expression of 4EHPGL01035 completely does not suppress the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

Expression of picoGD7437 does not suppress the eye degeneration seen when Atg1Scer\UAS.T:Hsap\MYC and Atg13Scer\UAS.T:Avic\GFP-EGFP are co-expressed under the control of Scer\GAL4GMR.PU at 18[o]C.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Atg13Scer\UAS.T:Avic\GFP-EGFP
Atg13UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (3)