FlyBase Allele Report: Dmel\C1GalTA[2.1]

General Information

Symbol

Dmel\C1GalTA^2.1

Species

D. melanogaster

Name

FlyBase ID

FBal0221560

Feature type

allele

Associated gene

Dmel\C1GalTA

Associated Insertion(s)

P{?SUPor-P}C1GalTA^2.1

Carried in Construct

Also Known As

dC1GalT1^2.1

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Lin et al., 2008)

Progenitor genotype

P{SUPor-P}C1GalTA^KG02976

(Lin et al., 2008)

Associated Insertion(s)

P{?SUPor-P}C1GalTA^2.1

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 6127bp deletion which removes much of the C1GalTA coding region, including the transmembrane domain and motifs predicted to be essential for its catalytic activity. 1584bp of the original insertion also remains.

(Lin et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

basement membrane | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

embryonic/larval plasmatocyte | embryonic stage 12

(Valoskova et al., 2019)

endocytic vesicle | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

germ band | embryonic stage 12

(Valoskova et al., 2019)

larval brain

(Lin et al., 2008)

larval ventral nerve cord

(Lin et al., 2008)

myofibril | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

NMJ bouton | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

postsynaptic density | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

sarcomere | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

subsynaptic reticulum | larval stage (with C1GalTA^EY13370)

(Itoh et al., 2016)

yolk | embryonic stage 12

(Valoskova et al., 2019)

Detailed Description

Statement

Reference

Stage 12 C1GalTA^2.1 embryos exhibit significantly fewer macrophages within the germ band and significantly more macrophages on the yolk, as compared to controls; the sum of the macrophages in the yolk and germband is the same in the controls.

(Valoskova et al., 2019)

C1GalTA^EY13370/C1GalTA^2.1 mutant larvae exhibit partial loss of the basement membrane, along with mislocalized neuromuscular junction boutons at the muscle 6/7 boundary, but no difference in the overall number of NMJ boutons, as compared to controls.

NMJs in C1GalTA^EY13370/C1GalTA^2.1 mutant larvae exhibit increased numbers of endocytic vesicles, increased thickness of subsynaptic reticulum per bouton area, decreased subsynaptic reticulum density, and decreased postsynaptic density length, but no significant difference in the number of synaptic vesicles, postsynaptic densities per bouton, or postsynaptic depth, as compared to wild type. Muscle fibers in C1GalTA^EY13370/C1GalTA^2.1 mutant larvae exhibit ectopic vacuole-like structures around the nucleus in myofibrils, and defective sarcomere structure, duplicate basement membranes, and increased basement membrane thickness, as compared to controls.

(Itoh et al., 2016)

Homozygous larvae have a greatly elongated ventral nerve cord (VNC) compared to controls, and the larval brain hemispheres are distorted. VNC condensation does occur in the homozygous embryos, but fails to reach the same extent as that seen in wild type, as the VNC is obviously elongated in first instar larvae. At each larval instar, the VNC of mutant animals is elongated compared to controls, and it continues to increase in length throughout larval development in the mutants. The elongated VNC of the mutant larvae is noticeably thinner than that of wild type, and is also more fragile when dissected.

(Lin et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

C1GalTA[+]/C1GalTA^2.1, GlcAT-P^SK6 has muscle cell of abdominal 3 ventral longitudinal muscle 3 phenotype

(Itoh et al., 2018)

C1GalTA[+]/C1GalTA^2.1, GlcAT-P^SK6 has muscle cell of abdominal 3 ventral longitudinal muscle 4 phenotype

(Itoh et al., 2018)

C1GalTA[+]/C1GalTA^2.1, GlcAT-P^SK6 has basement membrane | larval stage phenotype

(Itoh et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The muscles 6 and 7 at abdominal segment 3 of GlcAT-P^SK6, C1GalTA^2.1 double heterozygotes are frequently in close contact, covered with a continuous single basement membrane and/or show loss of basement membrane (assessed by Ndg protein levels), as compared to controls.

(Itoh et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

C1GalTA^2.1/C1GalTA^EY13370 is rescued by C1GalTA^UAS.cFa

(Itoh et al., 2016)

C1GalTA^2.1 is rescued by C1GalTA^+tLa

(Lin et al., 2008)

Comments

Expression of C1GalTA^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PU rescues the basement membrane loss of C1GalTA^EY13370/C1GalTA^2.1 mutants.