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General Information
Symbol
Dmel\C1GalTA2.1
Species
D. melanogaster
Name
FlyBase ID
FBal0221560
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dC1GalT12.1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 6127bp deletion which removes much of the C1GalTA coding region, including the transmembrane domain and motifs predicted to be essential for its catalytic activity. 1584bp of the original insertion also remains.

Insertion components
P{?SUPor-P}C1GalTA2.1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Stage 12 C1GalTA2.1 embryos exhibit significantly fewer macrophages within the germ band and significantly more macrophages on the yolk, as compared to controls; the sum of the macrophages in the yolk and germband is the same in the controls.

C1GalTAEY13370/C1GalTA2.1 mutant larvae exhibit partial loss of the basement membrane, along with mislocalized neuromuscular junction boutons at the muscle 6/7 boundary, but no difference in the overall number of NMJ boutons, as compared to controls.

NMJs in C1GalTAEY13370/C1GalTA2.1 mutant larvae exhibit increased numbers of endocytic vesicles, increased thickness of subsynaptic reticulum per bouton area, decreased subsynaptic reticulum density, and decreased postsynaptic density length, but no significant difference in the number of synaptic vesicles, postsynaptic densities per bouton, or postsynaptic depth, as compared to wild type. Muscle fibers in C1GalTAEY13370/C1GalTA2.1 mutant larvae exhibit ectopic vacuole-like structures around the nucleus in myofibrils, and defective sarcomere structure, duplicate basement membranes, and increased basement membrane thickness, as compared to controls.

Homozygous larvae have a greatly elongated ventral nerve cord (VNC) compared to controls, and the larval brain hemispheres are distorted. VNC condensation does occur in the homozygous embryos, but fails to reach the same extent as that seen in wild type, as the VNC is obviously elongated in first instar larvae. At each larval instar, the VNC of mutant animals is elongated compared to controls, and it continues to increase in length throughout larval development in the mutants. The elongated VNC of the mutant larvae is noticeably thinner than that of wild type, and is also more fragile when dissected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The muscles 6 and 7 at abdominal segment 3 of GlcAT-PSK6, C1GalTA2.1 double heterozygotes are frequently in close contact, covered with a continuous single basement membrane and/or show loss of basement membrane (assessed by Ndg protein levels), as compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of C1GalTAScer\UAS.cFa under the control of Scer\GAL4Act5C.PU rescues the basement membrane loss of C1GalTAEY13370/C1GalTA2.1 mutants.

Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer

Separable from: a secondary lethal mutation that results in embryonic lethality.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)