Gr93a3 mutant flies do not show significant changes in the expected avoidance response to food laced with acetic acid (5%), as compared to controls.
Gr93a3 mutant adults display impaired avoidance of umbelliferone in a two-way choice feeding assay compared to wild-type as well as reduced frequency of action potentials invoked upon exposure to umbelliferone (measured on S3, S5, S6 and S9 sensillae).
Gr93a3 mutant females display impaired umbelliferone avoidance in an assay testing site selection for egg laying but show normal aversion to lobeline as well as quinine.
Gr93a3 mutant larvae, like the wild-type, show preference for sucrose containing media over agarose-only in a binary choice larval behavior assay but unlike the wild-type keep preferring the sucrose media even if contaminated with umbelliferone.
Mutant flies show normal L-canavanine avoidance in a two-way choice test.
Gr93a3 flies show a similar preference for sugars (maltose, trehalose, fructose, glucose or sucrose) as wild-type controls in a binary food-choice assay between 1% agarose plus 10mM sugar and 1% agarose only.
Gr93a3 flies avoid quinine, strychnine, berberine, denatonium, lobeline and papaverine to the same extent as wild-type in a binary food-choice assay between 1mM sucrose alone and 5mM sucrose plus aversive compound. Action potentials induced in the gustatory receptor neurons in response to these aversive compounds are similar in Gr93a3 and wild-type animals.
Gr93a3 flies show reduced avoidance to caffeine in a binary food-choice assay. Caffeine-induced action potentials are virtually eliminated in the gustatory receptor neurons in Gr93a3 animals.
Mutant flies show reduced avoidance to theophylline in a binary food-choice assay, although avoidance responses to theobromine and paraxanthine are normal. Theophylline-induced action potentials are virtually eliminated in the gustatory receptor neurons in the mutant animals, although theobromine- or paraxanthine-induced action potentials are normal.
Gr93a3 is partially rescued by Gr93aUAS.cPa/Scer\GAL4Gr66a.PD
Gr93a3 is partially rescued by Scer\GAL4Gr66a.PD/Gr93aUAS.Tag:MYC
Expression of Gr93aScer\UAS.cPa under the control of Scer\GAL4Gr66a.PD in the Gr93a3 homozygote adults rescues the perturbed avoidance of umbelliferone in a two-way choice feeding assay as well as the site selection for oviposition assay and partially restores the reduction in frequency of action potentials invoked in the S6 sensilla upon umbelliferone exposure.
The Scer\GAL4 coding region inserted in Gr93a3 is not expressed.