FlyBase Allele Report: Dmel\nopo[5-SZ-3004]

General Information

Symbol

Dmel\nopo^5-SZ-3004

Species

D. melanogaster

Name

FlyBase ID

FBal0222051

Feature type

allele

Associated gene

Dmel\nopo

Associated Insertion(s)

P{RS5}nopo^5-SZ-3004

Carried in Construct

Also Known As

nopo^SZ3004

Key Links

Genomic Maps

JBrowse

Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

heat sensitive hypomorphic allele - genetic evidence

(Merkle et al., 2009)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{RS5}nopo^5-SZ-3004

Cytology

Description

Insertion in the 5' UTR.

(Merkle et al., 2009)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:18,180,663..18,180,663 [-]

(Ma et al., 2012, Merkle et al., 2009, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitotic spindle | maternal effect

(Wallace et al., 2014)

Detailed Description

Statement

Reference

Embryos derived from homozygous nopo^5-SZ-3004 females have a reduced hatching rate of approximately 40%. Mitotic spindle defects are seen in more than 40% of these embryos. The embryos are developmentally delayed, with only 13-15% reaching gastrulation by 5 hours after egg deposition.

(Wallace et al., 2014)

At 25[o]C, 62% of embryos derived from homozygous females mated to wild-type males develop to cycle 10 or beyond, 65% develop to the initiation of gastrulation or beyond, and 12% hatch.

At 18[o]C, 78% of embryos derived from homozygous females mated to wild-type males develop to cycle 10 or beyond, 86% develop to the initiation of gastrulation or beyond, and 76% hatch.

At 25[o]C, 67% of embryos derived from nopo^Z1447/Df(2R)Exel7153 females mated to wild-type males develop to cycle 10 or beyond, 12% develop to the initiation of gastrulation or beyond, but none of them hatch.

(Merkle et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nopo^5-SZ-3004 has partially lethal | maternal effect phenotype, suppressible | maternal effect by Scer\GAL4^{VP16.nanos.UTR}/PolH^UASp.Tag:HA

(Wallace et al., 2014)

nopo^5-SZ-3004 has majority die during embryonic stage | maternal effect phenotype, suppressible | maternal effect by Scer\GAL4^{VP16.nanos.UTR}/PolH^UASp.Tag:HA

(Wallace et al., 2014)

nopo^5-SZ-3004 has abnormal developmental rate | maternal effect phenotype, suppressible | maternal effect by Scer\GAL4^{VP16.nanos.UTR}/PolH^UASp.Tag:HA

(Wallace et al., 2014)

Suppressor of

Statement

Reference

nopo^5-SZ-3004 is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Ma et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

nopo^5-SZ-3004 has mitotic spindle | maternal effect phenotype, suppressible | maternal effect by Scer\GAL4^{VP16.nanos.UTR}/PolH^UASp.Tag:HA

(Wallace et al., 2014)

Suppressor of

Statement

Reference

nopo^5-SZ-3004 is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Ma et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The developmental delay, mitotic spindle defects and reduced hatching rate of embryos derived from homozygous nopo^5-SZ-3004 females is suppressed by expression of DNApol-η^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Wallace et al., 2014)

A nopo^5-SZ-3004 mutant background partially suppresses the small eye phenotype found in flies expressing egr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PF.

(Ma et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the insertion restores the hatch rates of embryos derived from females.

(Merkle et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nopo^5-SZ-3004

nopo^SZ3004

(Wallace et al., 2014, Merkle et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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