FlyBase Allele Report: Dmel\Drp1[UAS.Tag:HA]

General Information

Symbol

Dmel\Drp1^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0230285

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

P{UAS-Drp1.HA}

Also Known As

UAS-drp1

Key Links

Transgenic product class

wild_type

(Park et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Park et al., 2009)

Mutagen

in vitro construct

(Park et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Drp1.HA}

Cytology

Description

UAS regulatory sequences drive expression of Drp1 which is tagged at the C-terminal end with Tag:HA.

(Park et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Drp1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate Charcot-Marie-Tooth disease type 2

modeled by Marf^R94Qlike.UAS

(El Fissi et al., 2018)

ameliorates Charcot-Marie-Tooth disease type 2

modeled by Marf^{R364Wlike.UAS}

(El Fissi et al., 2018)

modeled by Marf^L76Plike.UAS

(El Fissi et al., 2018)

ameliorates hereditary spastic paraplegia 61

modeled by Arl6IP1^KK109154

(Fowler et al., 2020)

modeled by Arl6IP1^GD3027

(Fowler et al., 2020)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Terriente-Felix et al., 2020)

ameliorates Parkinson's disease 2

modeled by park²⁵

(Terriente-Felix et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

Phenotype Manifest In

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

Detailed Description

Statement

Reference

Expression of Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF leads to an increase in sensitivity to rotenone toxicity, with an increase in the number of dopaminergic neurons lost from the PPL1 cluster in adult brains after rotenone feeding, as compared to controls.

Flies expressing Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU do not exhibit any discernible eye phenotype, as compared to controls.

Dopaminergic neurons in flies expressing Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF do not exhibit obvious mitochondrial fragmentation, as compared to controls.

(Hwang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Drp1^UAS.Tag:HA, Scer\GAL4^ple.PF has chemical sensitive | adult stage phenotype, suppressible by Hsap\UCP2^UAS.cFa, Scer\GAL4^ple.PF

(Hwang et al., 2014)

NOT Enhancer of

Statement

Reference

Drp1^UAS.Tag:HA, Scer\GAL4^elav.PU is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^elav.PU

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a non-enhancer of abnormal size | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^da.G32/Drp1^UAS.Tag:HA is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32/Drp1^UAS.Tag:HA is a suppressor of abnormal locomotor behavior | adult stage phenotype of park²⁵

(Terriente-Felix et al., 2020)

Drp1^UAS.Tag:HA, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^elav.PU

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a suppressor | partially of abnormal size | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a suppressor | partially of decreased cell number | adult stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Drp1^UAS.Tag:HA, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Marf^L76Plike.UAS, Scer\GAL4^elav.PU

(El Fissi et al., 2018)

Scer\GAL4^Mef2.PR/Drp1^UAS.Tag:HA is a suppressor of abnormal flight phenotype of porin^A2

(Park et al., 2010)

Drp1^UAS.Tag:HA/Scer\GAL4^hs.PB is a suppressor of visible phenotype of Pink1^B9

(Park et al., 2009)

Drp1^UAS.Tag:HA/Scer\GAL4^hs.PB is a suppressor of flightless phenotype of Pink1^B9

(Park et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.G32, Drp1^UAS.Tag:HA, fwd^GL00145 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of park²⁵

(Terriente-Felix et al., 2020)

Drp1^UAS.Tag:HA, Scer\GAL4^da.G32 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^da.G32, fwd^GL00145

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32, Drp1^UAS.Tag:HA, fwd^GL00145 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Terriente-Felix et al., 2020)

Drp1^UAS.Tag:HA, Scer\GAL4^elav.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^elav.PU

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a non-suppressor of abnormal size | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Drp1^UAS.Tag:HA, Scer\GAL4^ple.PF has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by Hsap\UCP2^UAS.cFa, Scer\GAL4^ple.PF

(Hwang et al., 2014)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a non-enhancer of mitochondrion | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a non-enhancer of embryonic/larval motor neuron | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^da.G32/Drp1^UAS.Tag:HA is a suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32/Drp1^UAS.Tag:HA is a suppressor of flight muscle cell phenotype of Pink1^B9

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32/Drp1^UAS.Tag:HA is a suppressor of mitochondrion | adult stage phenotype of park²⁵

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32/Drp1^UAS.Tag:HA is a suppressor of flight muscle cell phenotype of park²⁵

(Terriente-Felix et al., 2020)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a suppressor | partially of mitochondrion | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a suppressor | partially of embryonic/larval motor neuron | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a suppressor | partially of eye photoreceptor cell phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a suppressor of NMJ bouton | larval stage phenotype of Marf^{R364Wlike.UAS}, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^Mef2.PR/Drp1^UAS.Tag:HA is a suppressor of mitochondrion phenotype of porin^A2

(Park et al., 2010)

Scer\GAL4^Mef2.PR/Drp1^UAS.Tag:HA is a suppressor of indirect flight muscle cell phenotype of porin^A2

(Park et al., 2010)

Drp1^UAS.Tag:HA/Scer\GAL4^hs.PB is a suppressor of wing phenotype of Pink1^B9

(Park et al., 2009)

Drp1^UAS.Tag:HA/Scer\GAL4^hs.PB is a suppressor of adult thorax phenotype of Pink1^B9

(Park et al., 2009)

Scer\GAL4^ple.PF/Drp1^UAS.Tag:HA is a suppressor of mitochondrion & dopaminergic neuron phenotype of Pink1^B9

(Park et al., 2009)

Drp1^UAS.Tag:HA/Scer\GAL4^hs.PB is a suppressor of wing phenotype of park¹

(Park et al., 2009)

Drp1^UAS.Tag:HA/Scer\GAL4^hs.PB is a suppressor of adult thorax phenotype of park¹

(Park et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.G32, Drp1^UAS.Tag:HA, fwd^GL00145 is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32, Drp1^UAS.Tag:HA, fwd^GL00145 is a non-suppressor of flight muscle cell phenotype of Pink1^B9

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32, Drp1^UAS.Tag:HA, fwd^GL00145 is a non-suppressor of mitochondrion | adult stage phenotype of park²⁵

(Terriente-Felix et al., 2020)

Scer\GAL4^da.G32, Drp1^UAS.Tag:HA, fwd^GL00145 is a non-suppressor of flight muscle cell phenotype of park²⁵

(Terriente-Felix et al., 2020)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a non-suppressor of mitochondrion | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Scer\GAL4^elav.PU/Drp1^UAS.Tag:HA is a non-suppressor of embryonic/larval motor neuron | larval stage phenotype of Marf^R94Qlike.UAS, Scer\GAL4^VGlut1-OK371

(El Fissi et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The defect in mitochondrial morphology in the indirect flight muscles which is seen in porin^A2 homozygotes is suppressed by expression of Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Mef2.PR.

The defects in flight ability that are seen in porin^A2 flies 20 days after eclosion are rescued by expression of Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Mef2.PR.

(Park et al., 2010)

The downturned wing, crushed thorax and flightless phenotypes of Pink1^B9 mutant flies are suppressed by expression of Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.PB.

The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1^B9 mutants is suppressed by expression of Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF.

(Park et al., 2009)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\UCP2^Scer\UAS.cFa suppresses the increased rotenone sensitivity seen in dopaminergic neurons of flies expressing Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ple.PF.

Co-expression of Hsap\UCP2^Scer\UAS.cFa and Drp1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU does not result in a rough eye phenotype.

(Hwang et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

95248

w^*; P{UAS-Drp1.HA}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Drp1^{Scer\UAS.T:Ivir\HA1}

Drp1^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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