FlyBase Allele Report: Dmel\cac[KK101478]

General Information

Symbol

Dmel\cac^KK101478

Species

D. melanogaster

Name

FlyBase ID

FBal0231294

Feature type

allele

Associated gene

Dmel\cac

Associated Insertion(s)

Carried in Construct

P{KK101478}

Also Known As

cac RNAi, UAS-Cac-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101478}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101478

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by zyd¹

(Weiss et al., 2019)

ameliorates hyperuricemia

modeled by Uro^NIG.7171R

(Hilsabeck et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with Scer\GAL4^tim.PE

(Hidalgo et al., 2021)

abnormal locomotor behavior | adult stage, with Scer\GAL4^tim.PE

(Hidalgo et al., 2021)

abnormal neurophysiology, with Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Brusich et al., 2015)

abnormal neurophysiology, with Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Brusich et al., 2015)

abnormal sleep | adult stage, with Scer\GAL4^tim.PE

(Hidalgo et al., 2021)

decreased efficacy of medium-term memory | adult stage, with Scer\GAL4^c309

(Hidalgo et al., 2021)

decreased efficacy of medium-term memory | adult stage, with Scer\GAL4^ey-OK107

(Hidalgo et al., 2021)

decreased efficacy of short-term memory | adult stage, with Scer\GAL4^c309

(Hidalgo et al., 2021)

decreased efficacy of short-term memory | adult stage, with Scer\GAL4^ey-OK107

(Hidalgo et al., 2021)

lethal - all die before end of P-stage, with Scer\GAL4^elav-C155, cac^HC129, cac^UAS.EGFP

(Brusich et al., 2015)

some die during pupal stage, with Scer\GAL4^elav-C155, cac^HC129, cac^UAS.EGFP

(Brusich et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Pre-synaptic expression of cac^KK101478 under the control of Scer\GAL4^elav-C155 and Scer\GAL4^sca.PC diminishes evoked transmission at the larval NMJ compared with wild type controls. Muscle expression of cac^KK101478 (under the control of Scer\GAL4^C57) has no effect on neurotransmission.

Expression of cac^KK101478 under the control of multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57) results in markedly decreased NMJ-evoked excitation and quantal content compared to controls.

(Brusich et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC, cac^KK101478 has abnormal neurophysiology phenotype, enhanceable by GluRIIC^RNAi.UAS.cBa, Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Brusich et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of GluRIIC^{dsRNA.Scer\UAS.cBa} enhances the reduction in quantal size seen in the larval NMJ when cac^KK101478 is expressed in multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57) alone. However, the defects in evoked transmission are not enhanced due to a compensatory increase in presynaptic quantal content.

(Brusich et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v104168

P{KK101478}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cac^KK101478

(Brusich et al., 2015)

cac^RNAi

(Weiss et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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