FlyBase Allele Report: Dmel\phtm[KK103215]

General Information

Symbol

Dmel\phtm^KK103215

Species

D. melanogaster

Name

FlyBase ID

FBal0232232

Feature type

allele

Associated gene

Dmel\phtm

Associated Insertion(s)

Carried in Construct

P{KK103215}

Also Known As

UAS-phm-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK103215}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK103215

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^P2.4.Pdf

(Itoh et al., 2011)

abnormal developmental rate | first instar larval stage, with Scer\GAL4^phtm.PO

(Okamoto et al., 2018)

female semi-fertile, with Scer\GAL4^GMR47A04

(Deady et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Knockdown of phm^KK103215 driven by Scer\GAL4^GMR47A04 causes a significant reduction in egg laying compared to wild type.

(Deady et al., 2015)

Over half of flies over-expressing phm^KK103215 under the control of Scer\GAL4^P2.4.Pdf exhibit circadian rhythm arrhythmia, with the remaining flies exhibiting a slightly longer period.

(Itoh et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^phtm.PO, phtm^KK103215 has abnormal developmental rate | first instar larval stage phenotype, non-enhanceable by Oatp74D²/Oatp74D¹

(Okamoto et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^phtm.PO, phtm^KK103215 has abnormal developmental rate | first instar larval stage phenotype, non-suppressible by Oatp74D²/Oatp74D¹

(Okamoto et al., 2018)

NOT Enhancer of

Statement

Reference

phtm^KK103215/Scer\GAL4^phtm.PO is a non-enhancer of abnormal developmental rate | first instar larval stage phenotype of Oatp74D²/Oatp74D¹

(Okamoto et al., 2018)

NOT Suppressor of

Statement

Reference

phtm^KK103215/Scer\GAL4^phtm.PO is a non-suppressor of abnormal developmental rate | first instar larval stage phenotype of Oatp74D²/Oatp74D¹

(Okamoto et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108359

P{KK103215}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

phm^KK103215

phm^RNAi

(Deady et al., 2015)

phtm^KK103215

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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