FlyBase Allele Report: Dmel\dome[KK104700]

General Information

Symbol

Dmel\dome^KK104700

Species

D. melanogaster

Name

FlyBase ID

FBal0233143

Feature type

allele

Associated gene

Dmel\dome

Associated Insertion(s)

Carried in Construct

P{KK104700}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK104700}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK104700

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

abnormal size | third instar larval stage, with Scer\GAL4^en-e16E

(Recasens-Alvarez et al., 2017)

abnormal size | third instar larval stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

visible | adult stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

Phenotype Manifest In

egg chamber, with Scer\GAL4^NP1624

(Berns et al., 2014)

epithelium, with Scer\GAL4^NP1624

(Berns et al., 2014)

mesonotum | adult stage | ectopic, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

mesothoracic tergum primordium | ectopic | third instar larval stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

stalk follicle cell, with Scer\GAL4^NP1624

(Berns et al., 2014)

wing, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

wing disc | third instar larval stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en-e16E

(Recasens-Alvarez et al., 2017)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

wing pouch | third instar larval stage, with Scer\GAL4^sd.PU

(Recasens-Alvarez et al., 2017)

Detailed Description

Statement

Reference

The expression of dome^KK104700 under the control of Scer\GAL4^sd.PU (and Dicer-2, for more efficient RNAi) leads to a high prevalence of vestigial or absent wings and/or a reciprocal duplications of mesonotum structures, associated with the frequent decrease/loss of the wing field primordium and/or a reciprocal mesonotum primordia duplication in third instar larval wing discs, as compared to controls; this Scer\GAL4^sd.PU-driven expression also results in third instar larval wing discs showing a severe size reduction, which is particularly evident in the posterior compartment, as compared to controls. The same severe posterior compartment size reduction is observed upon expression under the control of Scer\GAL4^en-e16E (and Dicer-2, for more efficient RNAi). Conversely, expression under the control of Scer\GAL4^ci.PU does not lead to any significant change in the size of the third instar larval anterior compartment, as compared to controls.

(Recasens-Alvarez et al., 2017)

Scer\GAL4^tj-NP1624-mediated expression results in 'fused' egg chambers in which individual chambers are covered by an epithelium but not separated by a stalk.

(Berns et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, dome^KK104700 has visible | adult stage phenotype, enhanceable by wg[+]/wg^l-17

(Recasens-Alvarez et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^sd.PU, dome^KK104700 has visible | adult stage phenotype, suppressible | partially by Egfr[+]/Egfr^f2

(Recasens-Alvarez et al., 2017)

Scer\GAL4^sd.PU, dome^KK104700 has visible | adult stage phenotype, suppressible by Df(3L)iro^EGP7/+

(Recasens-Alvarez et al., 2017)

NOT Suppressor of

Statement

Reference

dome^KK104700, Scer\GAL4^ap-md544 is a non-suppressor of abnormal developmental rate phenotype of BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544

(Romão et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, dome^KK104700 has mesonotum | adult stage | ectopic phenotype, enhanceable by wg[+]/wg^l-17

(Recasens-Alvarez et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^sd.PU, dome^KK104700 has mesonotum | ectopic | adult stage phenotype, suppressible | partially by Egfr[+]/Egfr^f2

(Recasens-Alvarez et al., 2017)

Scer\GAL4^sd.PU, dome^KK104700 has mesonotum | ectopic | adult stage phenotype, suppressible by Df(3L)iro^EGP7/+

(Recasens-Alvarez et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The highly prevalent duplication of mesanotum structures observed upon the expression of dome^KK104700 under the control of Scer\GAL4^sd.PU (and Dicer-2, for more efficient RNAi) is fully suppressed by Df(3L)iro^EGP7 heterozygosity and partially suppressed by Egfr^f2 heterozygosity, but is slightly enhanced by wg^l-17 heterozygosity.

The non-autonomous induction of ectopic wing structures in third instar larval wing discs resulting from the expression of dome^KK104700 under the control of Scer\GAL4^ci.PU is suppressed by the co-expression of dome^{ΔCYT.Scer\UAS}.

(Recasens-Alvarez et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106071

P{KK104700}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Domeless^RNAi

(Wang et al., 2015)

dome^KK104700

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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