FlyBase Allele Report: Dmel\RYBP[RNAi.UAS.cGa]

General Information

Symbol

Dmel\RYBP^RNAi.UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0239225

Feature type

allele

Associated gene

Dmel\RYBP

Associated Insertion(s)

Carried in Construct

P{UAS-RYBP.RNAi.G}

Key Links

Transgenic product class

RNAi_reagent

(González et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(González et al., 2008)

Mutagen

in vitro construct

(González et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-RYBP.RNAi.G}

Cytology

Description

UASt regulatory sequences drive expression of a RYBP RNAi construct that uses a 480bp RYBP fragment.

(González et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RYBP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

increased cell death, with Scer\GAL4^en.PU

(Fereres et al., 2013)

increased cell death, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

lethal | embryonic stage, with Scer\GAL4^en-e16E

(González et al., 2008)

lethal | larval stage, with Scer\GAL4^en-e16E

(González et al., 2008)

visible, with Scer\GAL4^ci.PC

(González et al., 2008)

visible, with Scer\GAL4^en-e16E

(González et al., 2008)

Phenotype Manifest In

ventral denticle belt, with Scer\GAL4^en-e16E

(González et al., 2008)

wing, with Scer\GAL4^ci.PC

(González et al., 2008)

wing, with Scer\GAL4^en.PU

(Fereres et al., 2013)

wing, with Scer\GAL4^en-e16E

(González et al., 2008)

wing, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

wing disc, with Scer\GAL4^en.PU

(Fereres et al., 2013)

wing disc, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

Detailed Description

Statement

Reference

Scer\GAL4^sd.PU-mediated expression of RYBP^{dsRNA.Scer\UAS.cGa} moderately reduces wing size.

Scer\GAL4^sd.PU- or Scer\GAL4^en.PU-mediated expression of RYBP^{dsRNA.Scer\UAS.cGa} weakly induces cell death in wing discs.

70% of flies expressing RYBP^{dsRNA.Scer\UAS.cGa} via Scer\GAL4^en.PU have wing blisters.

(Fereres et al., 2013)

Wing discs of Scer\GAL4^sd-SG29.1 RYBP^{dsRNA.Scer\UAS.cGa} larvae show similar levels of developmental apoptosis as wild type controls.

(González and Busturia, 2009)

Animals expressing RYBP^{dsRNA.Scer\UAS.cGa} under the control of Scer\GAL4^en-e16E show progressive lethality throughout embryonic and larval development (approximately 50% of the embryos die during embryogenesis).

Animals expressing RYBP^{dsRNA.Scer\UAS.cGa} under the control of Scer\GAL4^en-e16E that survive to secrete cuticle show an aberrant pattern of segmentation with severe disruption of the pattern of denticle belts, but no detectable homeotic transformations. The pattern of both the anterior and the posterior compartments is disrupted. This phenotype is highly variable in penetrance and expressivity.

Adult survivors expressing RYBP^{dsRNA.Scer\UAS.cGa} under the control of Scer\GAL4^en-e16E have wings which are blistered in the posterior compartment. The overall size of the wing is reduced, with a 45% reduction in the posterior compartment and a 35% reduction in the anterior compartment (the effect in the anterior compartment is non-autonomous).

Adult survivors expressing RYBP^{dsRNA.Scer\UAS.cGa} under the control of Scer\GAL4^ci.PC have wings which are blistered in the anterior compartment. The overall size of the wing is reduced.

Expression of RYBP^{dsRNA.Scer\UAS.cGa} under the control of one of Scer\GAL4^arm.PS, Scer\GAL4^da.G32 or Scer\GAL4^tub does not produce blistered wings.

Embryos expressing RYBP^{dsRNA.Scer\UAS.cGa} under the control of either Scer\GAL4^en-e16E or Scer\GAL4^ci.PC show a normal pattern of apoptosis.

(González et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU has abnormal size phenotype, enhanceable by slmb^HMS00946, Scer\GAL4^sd.PU

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU has abnormal size phenotype, enhanceable by SkpA^HM05185, Scer\GAL4^sd.PU

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^en.PU has increased cell death phenotype, enhanceable by SkpA^HM05185, Scer\GAL4^en.PU

(Fereres et al., 2013)

Enhancer of

Statement

Reference

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU is an enhancer of abnormal size phenotype of Scer\GAL4^sd.PU, SkpA^HM05185

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^en.PU is an enhancer of increased cell death phenotype of Scer\GAL4^en.PU, SkpA^HM05185

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU is an enhancer of abnormal size phenotype of Scer\GAL4^sd.PU, slmb^HMS00946

(Fereres et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU has wing phenotype, enhanceable by slmb^HMS00946, Scer\GAL4^sd.PU

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU has wing phenotype, enhanceable by SkpA^HM05185, Scer\GAL4^sd.PU

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^en.PU has wing disc phenotype, enhanceable by SkpA^HM05185, Scer\GAL4^en.PU

(Fereres et al., 2013)

Suppressed by

Statement

Reference

RYBP^RNAi.UAS.cGa, Scer\GAL4^en.PU has wing phenotype, suppressible by rpr^GD4698, Scer\GAL4^en.PU

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^en.PU has wing phenotype, suppressible | partially by Diap1^UAS.cUa, Scer\GAL4^en.PU

(Fereres et al., 2013)

Enhancer of

Statement

Reference

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU is an enhancer of wing phenotype of Scer\GAL4^sd.PU, SkpA^HM05185

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^en.PU is an enhancer of wing disc phenotype of Scer\GAL4^en.PU, SkpA^HM05185

(Fereres et al., 2013)

RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU is an enhancer of wing phenotype of Scer\GAL4^sd.PU, slmb^HMS00946

(Fereres et al., 2013)

Other

Statement

Reference

Cul1^GD9650, RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU has wing phenotype

(Fereres et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^sd.PU-mediated expression of both SkpA^HM05185 and RYBP^{dsRNA.Scer\UAS.cGa} reduces wing size to a greater extent than expression of either transgene alone.

Scer\GAL4^sd.PU-mediated expression of both slmb^HMS00946 and RYBP^{dsRNA.Scer\UAS.cGa} results in a more severe wing phenotype than expression of either transgene alone.

Scer\GAL4^sd.PU-mediated expression of both Cul1^GD9650 and RYBP^{dsRNA.Scer\UAS.cGa} results in flies completely lacking wing tissue.

Scer\GAL4^en.PU-mediated expression of both SkpA^HM05185 and RYBP^{dsRNA.Scer\UAS.cGa} increases cell death in the wing disc to a greater extent than expression of either transgene alone.

Co-expression of rpr^GD4698 completely suppresses the appearance of wing blisters in RYBP^{dsRNA.Scer\UAS.cGa} Scer\GAL4^en.PU flies.

Co-expression of Diap1^Scer\UAS.cUa reduces the penetrance of wing blisters in RYBP^{dsRNA.Scer\UAS.cGa} Scer\GAL4^en.PU flies.

(Fereres et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RYBP^RNAi.UAS.cGa

RYBP^{dsRNA.Scer\UAS.cGa}

RYBP^{dsRNA.UAS.cGa}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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