FlyBase Allele Report: Dmel\Rab1[JF02609]

General Information

Symbol

Dmel\Rab1^JF02609

Species

D. melanogaster

Name

FlyBase ID

FBal0239309

Feature type

allele

Associated gene

Dmel\Rab1

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02609}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.8.10)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.8.10)

Mutagen

in vitro construct

(Perkins et al., 2009.8.10)

Progenitor genotype

Carried in construct

P{TRiP.JF02609}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.8.10)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02609

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | prepupal stage P1 | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

abnormal neuroanatomy | pupal stage | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

Phenotype Manifest In

cis-Golgi network | third instar larval stage, with Scer\GAL4^Cg.PA

(Ke et al., 2018)

dendritic tree | prepupal stage P1 | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

dendritic tree | pupal stage | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

endoplasmic reticulum exit site | third instar larval stage, with Scer\GAL4^Cg.PA

(Ke et al., 2018)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

larval dorsal multidendritic neuron ddaC | prepupal stage P1 | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

Detailed Description

Statement

Reference

The expression of Rab1^JF02609 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body leads to defects in general secretion, as shown by the aberrant intracellular accumulation of Vkg.GFP (collagen IV), Trol.GFP, secr.GFP and Vkg.GFP.

The expression of Rab1^JF02609 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including extracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP. Expression under the control of Scer\GAL4^Cg.PA alone leads to enlarged endoplasmic reticulum exit sites (Sec16.GFP) and of the cis-Golgi (Grasp65.RFP).

(Ke et al., 2018)

The clonal expression of Rab1^JF02609 under the control of Scer\GAL4^ppk.PU in ddaC neuron leads to a significantly simplified dendrite arbors with fewer dendritic termini in white pre-pupae and leads to strong pruning defects in pupae (16h APF).

(Wang et al., 2018)

Expression of Rab1^JF02609 under the control of CtxGlia-SplitGal4 (Hsim\VP16^AD.nrv2 and Scer\GAL4^{DBD.wrapper.932i}) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

(Coutinho-Budd et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Rab1^JF02609, Scer\GAL4^{SPARC-MI00329-GAL4} is a suppressor of plasma membrane phenotype of Scer\GAL4^{SPARC-MI00329-GAL4}, cact^GL00627

(Zang et al., 2015)

Rab1^JF02609, Scer\GAL4^{SPARC-MI00329-GAL4} is a suppressor of plasma membrane phenotype of Scer\GAL4^{SPARC-MI00329-GAL4}, Tl^10b.UAS

(Zang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in plasma membrane depth seen in third larval instar adipocytes expressing either Tl^10b.Scer\UAS or cact^GL00627 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} is suppressed by co-expression of Rab1^JF02609.

(Zang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27299

y¹ v¹; P{TRiP.JF02609}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rab1^JF02609

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close