FlyBase Allele Report: Dmel\yki[S111A.S250A.UAS.Tag:V5]

General Information

Symbol

Dmel\yki^{S111A.S250A.UAS.Tag:V5}

Species

D. melanogaster

Name

FlyBase ID

FBal0239739

Feature type

allele

Associated gene

Dmel\yki

Associated Insertion(s)

Carried in Construct

P{UAS-yki.S111A.S250A.V5}

Key Links

Transgenic product class

missense_variant

(Oh and Irvine, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Oh and Irvine, 2009)

Mutagen

in vitro construct

(Oh and Irvine, 2009)

Progenitor genotype

Carried in construct

P{UAS-yki.S111A.S250A.V5}

Cytology

Description

Amino acid replacement: S111A.

(Oh and Irvine, 2009)

UAS regulatory sequences drive expression of a mutated form of yki which is tagged with Tag:V5.

(Oh and Irvine, 2009)

Amino acid replacement: S250A.

(Oh and Irvine, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

yki

Tagged with

Tag:V5

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

increased cell number | somatic clone, with Scer\GAL4^FRT.Act5C

(Oh and Irvine, 2011)

partially lethal, with Scer\GAL4^sd-SG29.1

(Oh and Irvine, 2009)

visible, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2009)

visible, with Scer\GAL4^sd-SG29.1

(Oh and Irvine, 2009)

visible | adult stage, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Oh and Irvine, 2011, Oh and Irvine, 2009)

wing, with Scer\GAL4^sd-SG29.1

(Oh and Irvine, 2009)

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

(Oh and Irvine, 2009)

wing disc | somatic clone, with Scer\GAL4^FRT.Act5C

(Oh and Irvine, 2011)

Detailed Description

Statement

Reference

Expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GMR.PF produces enlarged and irregular eyes.

Wing disc clones expressing yki^{S111A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^{Scer\FRT.Act5C} induce mild growth enhancement.

(Oh and Irvine, 2011)

Expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^GMR.PF results in an increase in the size of the eye and the eye is mostly flat.

Expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^sd-SG29.1 results in low viability and distorted wings.

Clones in the wing disc expressing yki^{S111A.S250A.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^Act5C.PI are rounder than normal.

(Oh and Irvine, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, yki^{S111A.S250A.UAS.Tag:V5} has visible | adult stage phenotype, enhanceable by Mad^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Scer\GAL4^GMR.PF, yki^{S111A.S250A.UAS.Tag:V5} has abnormal size | adult stage phenotype, enhanceable by Mad^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Scer\GAL4^GMR.PF, yki^{S111A.S250A.UAS.Tag:V5} has visible | adult stage phenotype, enhanceable by tkv^{Q253D.UAS.cNc}/Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Scer\GAL4^GMR.PF, yki^{S111A.S250A.UAS.Tag:V5} has abnormal size | adult stage phenotype, enhanceable by tkv^{Q253D.UAS.cNc}/Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Scer\GAL4^FRT.Act5C, yki^{S111A.S250A.UAS.Tag:V5} has increased cell number | somatic clone phenotype, enhanceable by tkv^{Q253D.UAS.cNc}/Mad^UAS.Tag:FLAG, Scer\GAL4^FRT.Act5C

(Oh and Irvine, 2011)

Enhancer of

Statement

Reference

yki^{S111A.S250A.UAS.Tag:V5}, Scer\GAL4^FRT.Act5C is an enhancer of increased cell number | somatic clone phenotype of Mad^UAS.Tag:FLAG, Scer\GAL4^FRT.Act5C, tkv^{Q253D.UAS.cNc}

(Oh and Irvine, 2011)

Other

Statement

Reference

Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S250A.UAS.Tag:V5} has visible phenotype

(Oh and Irvine, 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, yki^{S111A.S250A.UAS.Tag:V5} has eye phenotype, enhanceable by Mad^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Scer\GAL4^GMR.PF, yki^{S111A.S250A.UAS.Tag:V5} has eye phenotype, enhanceable by tkv^{Q253D.UAS.cNc}/Scer\GAL4^GMR.PF

(Oh and Irvine, 2011)

Scer\GAL4^FRT.Act5C, yki^{S111A.S250A.UAS.Tag:V5} has wing disc | somatic clone phenotype, enhanceable by tkv^{Q253D.UAS.cNc}/Mad^UAS.Tag:FLAG, Scer\GAL4^FRT.Act5C

(Oh and Irvine, 2011)

Enhancer of

Statement

Reference

yki^{S111A.S250A.UAS.Tag:V5}, Scer\GAL4^FRT.Act5C is an enhancer of wing disc | somatic clone phenotype of Mad^UAS.Tag:FLAG, Scer\GAL4^FRT.Act5C, tkv^{Q253D.UAS.cNc}

(Oh and Irvine, 2011)

Other

Statement

Reference

Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, yki^{S111A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^GMR.PF, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S250A.UAS.Tag:V5} has eye phenotype

(Oh and Irvine, 2009)

Scer\GAL4^Act5C.PI, hpo^UAS.Tag:FLAG, wts^UAS.Tag:MYC, yki^{S111A.S250A.UAS.Tag:V5} has wing disc | somatic clone phenotype

(Oh and Irvine, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mad^{Scer\UAS.T:Zzzz\FLAG} enhances the eye overgrowth seen when yki^{S111A.S250A.Scer\UAS.T:SV5\V5} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of tkv^{Q253D.Scer\UAS.cNc} enhances the eye overgrowth seen when yki^{S111A.S250A.Scer\UAS.T:SV5\V5} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5} enhances the overgrowth seen in wing disc clones expressing Mad^{Scer\UAS.T:Zzzz\FLAG} and tkv^{Q253D.Scer\UAS.cNc} under the control of Scer\GAL4^{Scer\FRT.Act5C}. The phenotype seen in stronger than is seen when yki^{S111A.S250A.Scer\UAS.T:SV5\V5} is expressed alone.

(Oh and Irvine, 2011)

Co-expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with small eyes.

Co-expression of yki^{S111A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in flies with very small eyes.

Clones in the wing disc co-expressing yki^{S111A.S250A.Scer\UAS.T:SV5\V5}, wts^{Scer\UAS.T:Hsap\MYC} and hpo^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PI do not survive well.

(Oh and Irvine, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

yki^{S111A.S250A.Scer\UAS.T:SV5\V5}

yki^{S111A.S250A.UAS.Tag:V5}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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