UASt regulatory sequences drive expression of the longest mtg isoform (this is the only predicted isoform to contain a secretion signal peptide) which is tagged with GFP.

Scer\GAL4^elav-C155/mtg^UAS.GFP fails to rescue mtg¹

50-90% of mtg¹ homozygous embryos are rescued to hatch by expression of mtg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32. The rescued larvae show coordinated locomotory movement and have a normal anatomy, including rescue of the cuticle defect seen in mtg¹ embryos. The majority of the rescued animals die in larval stages. On average, 50% of rescued animals that survive to the third larval instar ae fully rescued to adulthood, and overall up to 20% of mtg¹ homozygotes are completely rescued to adulthood by expression of mtg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32.

Expression of mtg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav-C155 does not rescue viability in mtg¹ homozygotes.

Expression of mtg^{Scer\UAS.T:Avic\GFP} under the simultaneous control of both Scer\GAL4^da.G32 and Scer\GAL4^elav-C155 increases full adult rescue of mtg¹ homozygotes by more than 3-fold at 18[o]C, compared to expression of mtg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 alone.

Expression of mtg^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^how-24B results in 18-32% hatching of mtg¹ homozygotes, however, all these larvae die within a few minutes of hatching. The cuticle herniation defect of mtg¹ embryos is rescued in these animals.