FlyBase Gene Report: Dmel\mtg

General Information

Symbol

Dmel\mtg

Species

D. melanogaster

Name

mind the gap

Annotation Symbol

CG7549

Feature Type

protein_coding_gene

FlyBase ID

FBgn0260386

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

mind the gap (mtg) encodes a protein involved in synapse assembly. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

84F6-84F6

Sequence location

3R:8,250,497..8,260,718 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:8250497..8260718

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables carbohydrate derivative binding

inferred from direct assay

(Rushton et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables chitin binding

inferred from electronic annotation with InterPro:IPR002557, InterPro:IPR036508

(InterPro Project Members, 2004-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in synapse assembly

inferred from direct assay

(Rohrbough et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Rushton et al., 2009)

located_in extracellular space

inferred from direct assay

(Rushton et al., 2012)

located_in synaptic cleft

inferred from direct assay

(Rohrbough et al., 2007)

located_in terminal bouton

inferred from direct assay

(Rohrbough et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extracellular region

inferred from electronic annotation with InterPro:IPR002557

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

CHITIN BINDING DOMAIN-CONTAINING PROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

mind the gap (mtg) encodes a protein involved in synapse assembly. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

CHITIN BINDING DOMAIN-CONTAINING PROTEINS -: Chitin binding domains are found in certain classes of chitin binding proteins and chitin-active enzymes. (Adapted from FBrf0108362.)

Summary (Interactive Fly)

secreted carbohydrate-binding protein involved in synapse assembly - modifier of signaling by the ligand Jelly belly and its receptor Alk

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mtg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0C4DHF5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.42

Gene model reviewed during 5.43

Gene model reviewed during 5.49

Gene model reviewed during 6.04

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mtg-RE

FBtr0310080

NM_169212

2399

556

mtg-RF

FBtr0310081

NM_141518

2279

515

mtg-RG

FBtr0344713

NM_001300302

2110

387

mtg-RH

FBtr0344714

NM_001043221

2233

387

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mtg-PE

FBpp0301781

62.4

556

8.60

FBpp0301782

58.0

515

8.14

FBpp0311046

43.4

387

7.36

FBpp0311047

43.4

387

7.36

Q0KIA5

NP_001036686

ABI31146

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

387 aa isoforms: mtg-PG, mtg-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mtg using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mtg_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.95

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic anal pad

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic esophagus

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic proventriculus

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval posterior spiracle

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 17

organism

(Rohrbough et al., 2007)

larval stage -- adult stage

organism

(Rohrbough et al., 2007)

Additional Descriptive Data

mtg is mainly detected in embryos from stage 12 to 16. Lower levels are seen in later embryos and in larvae with a transient peak at L2. Expression again peaks on the third day after pupariation. Higher levels are seen in adult heads than bodies.

(Rohrbough et al., 2007)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

larval central nervous system

(Rohrbough et al., 2007)

larval ventral nerve cord

(Rohrbough et al., 2007)

motor neuron of ventral midline of embryo

(Rohrbough et al., 2007)

embryonic/larval motor neuron

(Rohrbough et al., 2007)

larval somatic muscle cell

(Rohrbough et al., 2007)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Rushton et al., 2009)

located_in extracellular space

inferred from direct assay

(Rushton et al., 2012)

located_in synaptic cleft

inferred from direct assay

(Rohrbough et al., 2007)

located_in terminal bouton

inferred from direct assay

(Rohrbough et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mtg in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mtg

View exonic expression by tissue for Dmel\mtg

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0260386

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40970/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260386

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0260386

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260386

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7549

Flygut - An atlas of the Drosophila adult midgut

FBgn0260386

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mtg

Transgenic constructs containing regulatory region of mtg

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(3R)CA3

(Rohrbough et al., 2007)

Df(3R)Exel6147

(Ryder, 2004.4.26)

Df(3R)p712

(Szeged Stock Center, 1998-)

Gene not disrupted in

Df(3R)dsx11

(Rohrbough et al., 2007)

Df(3R)p-XT103

(Rohrbough et al., 2007)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

mtg^S084910b

lethal - all die before end of first instar larval stage | recessive

mtg^S084910b

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

mtg^UAS.GFP

lethal - all die before end of second instar larval stage, with Scer\GAL4^da.G32

mtg^RNAi.UAS

lethal - all die during embryonic stage

mtg¹

lethal - all die during embryonic stage (with mtg¹)

mtg^P2

lethal - all die during embryonic stage (with mtg^P2)

mtg¹

lethal - all die during embryonic stage | recessive

mtg^P2

partially lethal, with Scer\GAL4^da.G32

mtg^UAS.GFP

partially lethal, with Scer\GAL4^elav-C155, mtg¹

mtg^RNAi.UAS

partially lethal, with Scer\GAL4^elav-C155, mtg^RNAi.UAS

mtg¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mtg^GD12270

viable, with Scer\GAL4^Mhc.PW

mtg^RNAi.UAS

viable, with Scer\GAL4^pnr-MD237

mtg^GD12270

Other Phenotypes

Allele

abnormal neurophysiology

mtg¹

abnormal neurophysiology | embryonic stage

abnormal neurophysiology | embryonic stage (with Df(3R)CA3)

mtg¹

flightless, with Scer\GAL4^Mef2.PR

mtg^GD12270

paralytic | embryonic stage

mtg¹

some die during embryonic stage

mtg¹

some die during embryonic stage (with mtg¹)

mtg^P2

some die during embryonic stage (with mtg^P2)

mtg¹

some die during embryonic stage | recessive

mtg^P2

some die during first instar larval stage | recessive

mtg^S084910b

some die during larval stage, with Scer\GAL4^da.G32

mtg^UAS.GFP

some die during second instar larval stage, with Scer\GAL4^da.G32

mtg^RNAi.UAS

Phenotype manifest in

Allele

embryonic/first instar larval cuticle

mtg¹

neuromuscular junction

mtg¹

neuromuscular junction | embryonic stage

neuromuscular junction | embryonic stage (with Df(3R)CA3)

mtg¹

polysome & neuromuscular junction | embryonic stage (with Df(3R)CA3)

mtg¹

synaptic cleft (with Df(3R)CA3)

mtg¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\Y56A3A.6

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP001597

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mtg. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (17)

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

ckd

2 of 13

thw

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

mtg^NIG.7549R

ameliorates cancer

modeled by Ras85D^V12.cUa.UAS, dlg1^RNAi.UAS.cUa

(Lodge et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mtg - Rae1

experimental knowledge based

(Guruharsha et al., 2011)

mtg - eIF2α

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Neto

enhanceable

mtg

(Wang et al., 2018)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0260386

MIST (protein-protein) - An integrated Molecular Interaction Database

40970

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84F6-84F6

Sequence location

3R:8,250,497..8,260,718 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84F6-84F6

Limits computationally determined from genome sequence between P{PZ}grn⁰⁵⁹³⁰ and P{PZ}Mcm2^rL074

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84F11-84F16

(determined by in situ hybridisation)

(Szeged Stock Center, 1998-, Deak et al., 1997)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.8

(Comeron et al., 2012)

(Rushton et al., 2003)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

50582

y¹ v¹; P{TRiP.GLC01704}attP2

61992

y¹ v¹; P{TRiP.HMJ23618}attP40

2352

Df(3R)X3F, P{RP49}mtg^P2 e¹/MKRS

50491

y¹ w^*; Mi{MIC}mtg^MI08820

17697

w¹¹¹⁸; PBac{PB}mtg^c05288/TM6B, Tb¹

65897

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06159}attP40

NIG-Fly

7549R-1

P{NIG.7549R}1

HMJ23618

y¹ v¹; P{TRiP.HMJ23618}attP40/CyO

VDRC

v28059

w¹¹¹⁸; P{GD12270}v28059

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (11)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH13023

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0260386

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260386

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

mtg binds N-acetylglucosamine in a Ca[2+]-dependent manner.

(Rushton et al., 2012)

Loss of mtg greatly reduces overall activity-dependent transmission.

(Rohrbough and Broadie, 2010)

mtg is required to form the specialised transsynaptic matrix that links the presynaptic active zone with the post-synaptic glutamate receptor domain in the synapse.

(Rohrbough et al., 2007)

Mutants show dramatically impaired neurotransmission (98% reduced) exhibited at the embryonic neuromuscular junction. Ultrastructural analyses have revealed the loss of the highly structured extracellular matrix in the synaptic cleft of mutant neuromuscular junctions.

(Fergestad et al., 2001)

Relationship to Other Genes

Source for database merge of

Source for merge of: mtg l(3)S084910b CG7549

(Rohrbough et al., 2007)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

0849/10

(Deak et al., 1997)

CG7549

(Hosono et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2010.12.1, Perkins et al., 2009, Rushton et al., 2009, Minidorff et al., 2007, Rohrbough et al., 2007, Zhang et al., 2007)

MTG

(Chou et al., 2020, Scott and Panin, 2014)

Mtg

(Rushton et al., 2020, Rohrbough and Broadie, 2010)

l(3)S084910

l(3)S084910b

mtg

(Yue et al., 2020, Huylmans and Parsch, 2014, Sopko and Perrimon, 2013, Catalán et al., 2012, Rushton et al., 2012, Rynes et al., 2012, Rushton et al., 2009, Rohrbough et al., 2007)

Name Synonyms

CG7549

Mind the gap

(Rohrbough and Broadie, 2010)

Mind-the-Gap

(Scott and Panin, 2014, Rushton et al., 2012)

lethal (3) S084910b

mind the gap

(Rushton et al., 2009, Rohrbough et al., 2007, Fergestad et al., 2001)

Secondary FlyBase IDs

FBgn0060301
FBgn0028084
FBgn0037559

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40970

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0C4DHF5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0C4DHF5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0260386

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40970/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260386

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260386

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7549

MARRVEL_MODEL - MARRVEL (model organism gene)

40970

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0260386

DroID - A comprehensive database of gene and protein interactions.

FBgn0260386

DRSC - Results frm RNAi screens

FBgn0260386

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mtg_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0260386

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0260386

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7549

Flygut - An atlas of the Drosophila adult midgut

FBgn0260386

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031511

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

40970

References (46)

The following external sites may use different assemblies or annotations than FlyBase.

-0.95

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