FlyBase Allele Report: Dmel\AdoR[UAS.cDa]

General Information

Symbol

Dmel\AdoR^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Dolezelova

FlyBase ID

FBal0240164

Feature type

allele

Associated gene

Dmel\AdoR

Associated Insertion(s)

Carried in Construct

P{UAS-AdoR.D}

Also Known As

UAS-AdoR

Key Links

Transgenic product class

genomic_DNA

(Dolezelova et al., 2007)

wild_type

(Dolezelova et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Dolezelova et al., 2007)

wild_type

(Dolezelova et al., 2007)

Mutagen

in vitro construct

(Dolezelova et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-AdoR.D}

Cytology

Description

UASt regulatory sequences drive expression of a genomic fragment containing AdoR.

(Dolezelova et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

AdoR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^{Act5C.Switch.PR}

(Kucerova et al., 2012)

lethal, with Scer\GAL4^pnr-MD237

(Dolezelova et al., 2007)

lethal - all die before end of larval stage, with Scer\GAL4^Act5C.PP

(Dolezelova et al., 2007)

lethal - all die before end of P-stage, with Scer\GAL4^pros.PU

(Dolezelova et al., 2007)

lethal - all die before end of P-stage, with Scer\GAL4^sev.PU

(Dolezelova et al., 2007)

melanotic mass phenotype, with Scer\GAL4^30A

(Dolezelova et al., 2007)

melanotic mass phenotype, with Scer\GAL4^en-e16E

(Dolezelova et al., 2007)

some die during larval stage, with Scer\GAL4^Act5C.PP

(Dolezelova et al., 2007)

some die during pharate adult stage, with Scer\GAL4^pros.PU

(Dolezelova et al., 2007)

some die during pharate adult stage, with Scer\GAL4^sev.PU

(Dolezelova et al., 2007)

visible, with Scer\GAL4^30A

(Dolezelova et al., 2007)

visible, with Scer\GAL4^en-e16E

(Dolezelova et al., 2007)

visible, with Scer\GAL4^pnr-MD237

(Dolezelova et al., 2007)

Phenotype Manifest In

adult external abdomen, with Scer\GAL4^pnr-MD237

(Dolezelova et al., 2007)

melanotic mass, with Scer\GAL4^30A

(Dolezelova et al., 2007)

melanotic mass, with Scer\GAL4^en-e16E

(Dolezelova et al., 2007)

scutellar bristle, with Scer\GAL4^pnr-MD237

(Dolezelova et al., 2007)

scutellum, with Scer\GAL4^pnr-MD237

(Dolezelova et al., 2007)

wing, with Scer\GAL4^30A

(Dolezelova et al., 2007)

wing, with Scer\GAL4^en-e16E

(Dolezelova et al., 2007)

Detailed Description

Statement

Reference

Virtually no eclosed adults emerge upon expression of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^{Act5C.Switch.PR}. Microinjection of the adenosine receptor antagonist SCH58261 into third instar larvae can partially rescue the lethality and leads to the emergence of approximately 20% of the animals as adults.

(Kucerova et al., 2012)

Expression of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP results in lethality during the transition from the first to second larval instar.

Expression of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^sev.PJ or Scer\GAL4^pros.PJ results in late pupal lethality. The pharate adults survive for several hours when removed from the pupal case at the normal eclosion time, but are unable to move or spread their wings.

Expression of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^en-e16E results in flies with folded and blistered wings (100%) and melanotic tumours in the head capsule (80%).

Expression of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^30A results in flies with folded and blistered wings (100%) and melanotic tumours in the head capsule (50%).

Expression of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 results in flies with a reduced scutellum and cross post-scutellar bristles. Flies expressing two copies of AdoR^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 emerge very rarely (less than 5%), have a strong cleft in the thorax and complete loss of the scutellum and have defects in the melanization of abdominal cuticle.

(Dolezelova et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

AdoR^UAS.cDa, Gαs^UAS.cCa, Scer\GAL4^en-e16E has visible phenotype

(Dolezelova et al., 2007)

AdoR^UAS.cDa, Gαs^UAS.cCa, Scer\GAL4^en-e16E has lethal | pharate adult stage phenotype

(Dolezelova et al., 2007)

Phenotype Manifest In

Other

Statement

Reference

AdoR^UAS.cDa, Gαs^UAS.cCa, Scer\GAL4^en-e16E has wing phenotype

(Dolezelova et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Animals co-expressing G-sα60A^Scer\UAS.cCa and AdoR^Scer\UAS.cDa under the control of Scer\GAL4^en-e16E show 20% lethality as pharate adults, whereas expression of either G-sα60A^Scer\UAS.cCa or AdoR^Scer\UAS.cDa alone under the control of Scer\GAL4^en-e16E does not cause lethality. The flies that do emerge show strong wing deformation and melanisation.

(Dolezelova et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

AdoR^Scer\UAS.cDa

AdoR^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Dolezelova

Secondary FlyBase IDs

References (3)

Report Sections

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