FlyBase Allele Report: Dmel\lft[TG2]

General Information

Symbol

Dmel\lft^TG2

Species

D. melanogaster

Name

FlyBase ID

FBal0241155

Feature type

allele

Associated gene

Dmel\lft

Associated Insertion(s)

TI{TI}lft^TG2

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Mao et al., 2009)

Mutagen

recombination

(Mao et al., 2009)

I-SceI

(Mao et al., 2009)

FLPase

(Mao et al., 2009)

ends-out gene targeting

(Irvine, 2016.3.31)

gene targeting by homologous recombination

(Mao et al., 2009)

Progenitor genotype

Associated Insertion(s)

TI{TI}lft^TG2

Cytology

Description

lft has been replaced by a mini-white marker (w^+*).

(Irvine, 2016.3.31)

Deletion of the entire lft coding region.

(Mao et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Mao et al., 2009)

viable

(Mao et al., 2009)

visible

(Mao et al., 2009)

visible (with lft³⁷⁰⁹)

(Mao et al., 2009)

visible (with lft³⁷⁶²)

(Mao et al., 2009)

visible | recessive

(Hogan et al., 2011)

Phenotype Manifest In

wing

(Mao et al., 2009)

wing (with lft³⁷⁰⁹)

(Mao et al., 2009)

wing (with lft³⁷⁶²)

(Mao et al., 2009)

wing blade

(Hogan et al., 2011)

Detailed Description

Statement

Reference

Homozygous lft^TG2 wings display altered wing morphology and aberrant posterior ridges, but wild-type hair polarity.

(Hogan et al., 2011)

Homozygotes have a reduced wing length compared to controls. The average wing area is 82% of that in wild type, and the average distance between the anterior and the posterior crossveins is reduced to 59% of that in wild type.

lft^TG2/lft³⁷⁶² and lft³⁷⁰⁹/lft^TG2 flies have a reduced wing length compared to controls (the distance between the anterior and the posterior crossveins is reduced).

The orientation of wing hairs is not significantly affected in mutant flies.

(Mao et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

lft^TG2 has visible phenotype, suppressible | partially by Scer\GAL4^Tub.PU/Hsap\LIX1^UAS.Tag:FLAG

(Mao et al., 2009)

lft^TG2 has visible phenotype, suppressible by Hsap\LIX1L^UAS.Tag:FLAG/Scer\GAL4^Tub.PU

(Mao et al., 2009)

Other

Statement

Reference

lft^TG2, pk³⁰ has visible phenotype

(Hogan et al., 2011)

lft^TG2, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

ds^UAO71/Df(2L)ED94, lft^TG2 has lethal phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has visible phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has abnormal planar polarity phenotype

(Mao et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

lft^TG2 has wing phenotype, suppressible | partially by Scer\GAL4^Tub.PU/Hsap\LIX1^UAS.Tag:FLAG

(Mao et al., 2009)

lft^TG2 has wing phenotype, suppressible by Hsap\LIX1L^UAS.Tag:FLAG/Scer\GAL4^Tub.PU

(Mao et al., 2009)

Other

Statement

Reference

lft^TG2, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

ft¹, lft^TG2 has femur phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has posterior crossvein phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has tibia phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has tarsal segment phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has wing vein L2 phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has wing hair phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has wing vein | ectopic phenotype

(Mao et al., 2009)

ft¹, lft^TG2 has leg phenotype

(Mao et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

In lft^TG2, pk³⁰ double mutant wings, the pk³⁰ hair phenotype is modified to a more distal polarity in the anterior and distal posterior regions.

(Hogan et al., 2011)

The distance between the anterior and posterior crossveins is greatly reduced in ft¹ lft^TG2 double mutants. Additional phenotypes are seen in the double mutant that are not seen in either single mutant; the posterior crossvein is incomplete and the L2 vein is often both incomplete and associated with ectopic vein material.

ft¹ lft^TG2 double mutants have shorter legs than normal and individual leg segments (including the femur and tibia) are bother shorter and broader than normal (leg growth is only very subtly affected in either single mutant). The legs of the double mutant flies have only four tarsal segments instead of the usual five.

ft¹ lft^TG2 double mutants have obvious planar cell polarity phenotypes in the wings and legs (in contrast to either single mutant).

(Mao et al., 2009)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\LIX1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^tub partially rescues the wing phenotype of lft^TG2 mutants.

Expression of Hsap\LIX1L^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^tub rescues the wing phenotype of lft^TG2 mutants.

(Mao et al., 2009)

Complementation and Rescue Data

Rescued by

lft^TG2 is rescued by Scer\GAL4^Tub.PU/lft^UAS.Tag:FLAG

(Mao et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

44247

w^*; TI{TI}lft^TG2 P{neoFRT}40A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lft^TG2

(Hogan et al., 2011, Mao et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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