FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\CTCFGE24185
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General Information
Symbol
Dmel\CTCFGE24185
Species
D. melanogaster
Name
FlyBase ID
FBal0241197
Feature type
allele
Associated gene
Dmel\CTCF
Associated Insertion(s)
P{EP}CTCFGE24185
Carried in Construct
Also Known As
GE24185
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Mohan et al., 2007)
Progenitor genotype
Associated Insertion(s)
P{EP}CTCFGE24185
Cytology
Description

The insertion P{EP}CTCFGE24185 is found in the nucleotide triplet encoding for amino acid residue 158 in CTCF.

(Mohan et al., 2007)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal developmental rate

      (Kyrchanova et al., 2021)

      decreased size | adult stage | female

      (Kyrchanova et al., 2021)

      female semi-fertile

      (Gambetta and Furlong, 2018)

      female sterile (with CTCFC1)

      (Kyrchanova et al., 2021)

      homeotic

      (Gambetta and Furlong, 2018, Savitsky et al., 2016)

      homeotic (with CTCFy+1)

      (Savitsky et al., 2016)

      homeotic | adult stage

      (Kyrchanova et al., 2021)

      homeotic | adult stage (with CTCFKO)

      (Gambetta and Furlong, 2018)

      homeotic | adult stage | maternal effect (with CTCFKO)

      (Gambetta and Furlong, 2018)

      homeotic | maternal effect

      (Gambetta and Furlong, 2018)

      majority die during P-stage (with CTCFKO)

      (Gambetta and Furlong, 2018)

      partially lethal - majority die

      (Kyrchanova et al., 2021)

      partially lethal - majority die (with CTCFKO)

      (Gambetta and Furlong, 2018)

      partially lethal - majority live

      (Mohan et al., 2007)

      partially lethal - majority live (with CTCFattP.mCh)

      (Kyrchanova et al., 2021)

      partially lethal - majority live (with CTCFC1)

      (Kyrchanova et al., 2021)

      partially lethal - majority live (with CTCFC2)

      (Kyrchanova et al., 2021)

      partially lethal - majority live | maternal effect (with CTCFKO)

      (Gambetta and Furlong, 2018)

      some die during larval stage | maternal effect

      (Gambetta and Furlong, 2018)

      some die during pupal stage

      (Gambetta and Furlong, 2018)

      sterile (with CTCFattP.mCh)

      (Kyrchanova et al., 2021)

      sterile (with CTCFattP)

      (Kyrchanova et al., 2021)

      viable

      (Savitsky et al., 2016)

      viable (with CTCFy+1)

      (Savitsky et al., 2016)

      visible | adult stage

      (Kyrchanova et al., 2021)

      visible | adult stage (with CTCFattP.mCh)

      (Kyrchanova et al., 2021)

      visible | adult stage (with CTCFattP)

      (Kyrchanova et al., 2021)

      visible | homeotic

      (Mohan et al., 2007)

      visible | homeotic (with Df(3L)0463)

      (Mohan et al., 2007)
      Phenotype Manifest In

      abdomen | adult stage

      (Gambetta and Furlong, 2018)

      abdomen | adult stage (with CTCFKO)

      (Gambetta and Furlong, 2018)

      abdomen | adult stage | maternal effect

      (Gambetta and Furlong, 2018)

      abdomen | adult stage | maternal effect (with CTCFKO)

      (Gambetta and Furlong, 2018)

      abdominal segment 4

      (Mohan et al., 2007)

      abdominal segment 4 (with Df(3L)0463)

      (Mohan et al., 2007)

      abdominal segment 6

      (Savitsky et al., 2016)

      abdominal segment 6 (with CTCFy+1)

      (Savitsky et al., 2016)

      abdominal segment 7

      (Savitsky et al., 2016)

      abdominal segment 7 (with CTCFy+1)

      (Savitsky et al., 2016)

      abdominal segment 8

      (Savitsky et al., 2016)

      abdominal segment 8 (with CTCFy+1)

      (Savitsky et al., 2016)

      abdominal segment | adult stage

      (Gambetta and Furlong, 2018)

      abdominal segment | adult stage (with CTCFKO)

      (Gambetta and Furlong, 2018)

      abdominal segment | adult stage | maternal effect

      (Gambetta and Furlong, 2018)

      abdominal segment | adult stage | maternal effect (with CTCFKO)

      (Gambetta and Furlong, 2018)

      abdominal sternite

      (Savitsky et al., 2016)

      abdominal sternite 6

      (Mohan et al., 2007)

      abdominal sternite 6 (with Df(3L)0463)

      (Mohan et al., 2007)

      abdominal sternite 6 | adult stage

      (Kyrchanova et al., 2021)

      abdominal sternite 7 (with Df(3L)0463)

      (Mohan et al., 2007)

      abdominal sternite (with CTCFy+1)

      (Savitsky et al., 2016)

      abdominal sternite | adult stage

      (Kyrchanova et al., 2021)

      abdominal sternite bristle

      (Savitsky et al., 2016, Mohan et al., 2007)

      abdominal sternite bristle (with CTCFy+1)

      (Savitsky et al., 2016)

      abdominal sternite bristle (with Df(3L)0463)

      (Mohan et al., 2007)

      abdominal tergite

      (Savitsky et al., 2016)

      abdominal tergite 5

      (Mohan et al., 2007)

      abdominal tergite 5 (with Df(3L)0463)

      (Mohan et al., 2007)

      abdominal tergite 7

      (Mohan et al., 2007)

      abdominal tergite 7 & abdominal sternite bristle (with Df(3L)0463)

      (Mohan et al., 2007)

      abdominal tergite (with CTCFy+1)

      (Savitsky et al., 2016)

      adult external abdomen (with CTCFattP.mCh)

      (Kyrchanova et al., 2021)

      adult external abdomen (with CTCFattP)

      (Kyrchanova et al., 2021)

      adult external abdomen | adult stage

      (Kyrchanova et al., 2021)

      female genitalia | adult stage (with CTCFattP)

      (Kyrchanova et al., 2021)

      female genitalia | adult stage (with CTCFC2)

      (Kyrchanova et al., 2021)

      genitalia | adult stage

      (Kyrchanova et al., 2021)

      genitalia | adult stage (with CTCFattP.mCh)

      (Kyrchanova et al., 2021)

      genitalia | adult stage (with CTCFC1)

      (Kyrchanova et al., 2021)

      hypogynium | adult stage

      (Kyrchanova et al., 2021)

      male genitalia

      (Mohan et al., 2007)

      male genitalia (with CTCFy+1)

      (Savitsky et al., 2016)

      male genitalia (with Df(3L)0463)

      (Mohan et al., 2007)

      wing

      (Kyrchanova et al., 2021, Mohan et al., 2007)

      wing (with Df(3L)0463)

      (Mohan et al., 2007)
      Detailed Description
      Statement
      Reference

      CTCFGE24185 homozygotes mostly reach adulthood, with some dying as before adulthood; 90% of eggs laid by CTCFGE24185 homozygous mothers are unfertilized but the rare fertilized progress through development with near normal viability and developed into adults. CTCFKO/CTCFGE24185 transheterozygous adults and progeny from females progress through embryo-larva stages with relatively normal viability but only half or less progress from pupa to adulthood.

      CTCFGE24185 homozygous CTCFKO/CTCFGE24185 zygotic-only and maternal-only mutants also exhibit homeotic transformations in the abdomen.

      (Gambetta and Furlong, 2018)

      CTCFGE24185/CTCFGE24185 are viable and show posterior-to-anterior homeotic transformations of abdominal segments A8-A6: in males s6 (sternite of the sixth segment) has 3-13 (average 8) bristles; in females s7 has 8-15 (average 12) bristles, and t8 (tergite of the eighth segment) often displays a bunch of small bristles (0-17, average 8).

      CTCFy+1/CTCFGE24185 are viable and show posterior-to-anterior homeotic transformations of abdominal segments A8-A6: in males genitalia is rotated 30-180[o] (70% penetrance), s6 has 4-9 (average 7) bristles, and t7 is well developed; in females s7 has 8-12 (average 10) bristles, the shape of s7 is transformed toward s6 and bristles lose orientation, and t8 has a row of large bristles.

      (Savitsky et al., 2016)

      CTCFGE24185 homozygotes exhibit reduced viability (66%) and held-out wings. Male genitalia are rotated up to 30[o]. Males exhibit an A4 patchy pigmentation, partial loss of pigment in A5 tergite (indicating that A5 is nominally transformed in A4), eight bristles on the sixth sternite, and a small seventh tergite (10-20%). Embryos from F[[2]] homozygotes do not eclose.

      CTCFGE24185/Df(3L)0463 trans-heterozygotes show a highly penetrant mild (60[o]) held-out wing phenotype, various homeotic transformations and sterility in both males and females. Male genitalia are rotated by 40[o]-120[o]. Males exhibit patchy pigmentation in the A4 abdominal segment, partial loss of pigment in the A5 tergite, eight bristles on the sxith sternite, and a small seventh tergite. Females exhibit bristles on the A7 sternite that have lost orientation.

      (Mohan et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The majority of CTCFGE24185 Cp190En15 /CTCFGE24185 Cp190P1 double mutants die as pharate adults, with rare (2%) weak escapers that show homeotic transformation of abdominal segment A1 into A2 (less pronounced than in Cp190En15/Cp190P1) and A6-A8 (A6/7 transformations weaker than in CTCFGE24185/CTCFGE24185, though A8 transformation is enhanced): the shape of t1 is changed toward t2 though large bristles are rare, s1 usually shows no bristles, t1 bristles are more uniform than wild type; in males s6 has 0-4 (average 1) bristles, t7 is enlarged and often developed to only one side in 50% of males, t8 is well separated from t9 and usually visible with a bunch of large bristles, and genitalia is rotated 90-180[o] (100%); in females s7 has 5-11 (average 8) bristles, the shape of s7 is transformed towards s6 and bristles lose orientation, t8 always has 7-19 (average 13) large bristles (often forming two rows).

      (Savitsky et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Df(3L)0463/CTCFGE24185 is rescued by CTCF+t5084

      (Mohan et al., 2007)

      CTCFGE24185 is rescued by CTCF+t5084

      (Mohan et al., 2007)
      Comments

      The majority of CTCFGE24185 flies are fully rescued by the presence of CTCF+t.5084, with a few showing various degrees of held-out wings and are unable to walk properly. The rescued flies are fertile and can be used to establish a breeding stock.

      The majority of CTCFGE24185/Df(3L)0463 flies are fully rescued by the presence of CTCF+t.5084, with a few showing various degrees of held-out wings and are unable to walk properly. The rescued flies are fertile and can be used to establish a breeding stock.

      (Mohan et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CTCFGE24185
      (Zhang et al., 2024, Gambetta and Furlong, 2018, Savitsky et al., 2016, Bonchuk et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)