7 days old females carrying homozygous germline clones do not show defects in oogenesis.

Homozygous projection neuron (PN) neuroblast MARCM clones labelled using Scer\GAL4^GH146 show two dendrite morphogenesis defects. Firstly, the dendritic density in most glomeruli is drastically reduced compared to controls. Secondly, dendritic branches spill into incorrect glomerular classes.

Homozygous single-cell "DL1" projection neuron clones show stereotyped mistargeting defects in 17/25 cases examined; the dendrites innervate the DL glomerulus more sparsely than normal and also mistarget several additional glomeruli (VA7m, VC2, VA6, DL2d and DL5), all of which are partially innervated. In the remaining DL1 single cell projection neuron clones, the neurons spill their dendrites medially to adjacent glomeruli, mostly D and DL5.

Homozygous projection neuron MARCM clones labelled using Scer\GAL4^Mz19 show sparse innervation of the VA1d/DC3 glomeruli and the dendrites are incorrectly targeted to variable glomeruli such as DA1, VA2 and VM7. 4/5 clones target lPNs normally to DA1 with wild-type dendrite densities, while 1/5 show additional partial innervation of the adjacent DL3 glomerulus.

The axons of homozygous single-cell "DL1" projection neuron clones project into the lateral horn (as occurs in wild type), but the main branches do not reach the edge of the lateral horn. In addition, the dorsal branch of the lateral horn projection is shorter than normal or absent.

The dendrite defects seen in homozygous projection neuron neuroblast or DL1 single cell clones are manifested during development; dendritic elaboration within the proto-antennal lobe is extremely reduced in all neuroblast or DL1 single cell clones at 18 hours after puparium formation (APF). At 50 hours APF, the dendrites of the projection neuron clones are reduced in density compared to wild type and spill into lineage-inappropriate glomeruli. In addition, the stereotyped mistargeting of homozygous DL1 single cell clones is already evident at 50 hours APF.