FlyBase Allele Report: Dmel\Dysb[UAS.cDa]

General Information

Symbol

Dmel\Dysb^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Dickman

FlyBase ID

FBal0241760

Feature type

allele

Associated gene

Dmel\Dysb

Associated Insertion(s)

Carried in Construct

P{UAS-Dysb.D}

Also Known As

UAS-dysb

Key Links

Transgenic product class

wild_type

(Dickman and Davis, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dickman and Davis, 2009)

Mutagen

in vitro construct

(Dickman and Davis, 2009)

Progenitor genotype

Carried in construct

P{UAS-Dysb.D}

Cytology

Description

UAS regulatory sequences drive expression of Dysb.

(Dickman and Davis, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dysb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^elav-C155

(Dickman et al., 2012, Dickman and Davis, 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 in a Dysb^e01028/+ mutant background has no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls.

Expression of Dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 has no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls.

(Mullin et al., 2015)

Overexpression of dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 leads to potentiation of baseline release.

(Dickman et al., 2012)

In larvae expressing dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155, synaptic transmission at the neuromuscular junction is normal at low extracellular Ca[2+] (0.2 and 0.3mM) but is enhanced at relatively high extracellular Ca[2+] (0.5mM).

(Dickman and Davis, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dysb^UAS.cDa, Scer\GAL4^elav-C155 has abnormal neurophysiology phenotype, suppressible by P{GD12574}, Scer\GAL4^elav-C155

(Dickman et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155, Dysb[+], Dysb^UAS.cDa, Dysb^e01028 is a non-suppressor of abnormal neurophysiology | dominant phenotype of Blos1^EY06269

(Mullin et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 in a Blos1^EY06269/+; Dysb^e01028/+ mutant background has no effect on excitatory junction potential (EJP) amplitude or miniature EJP frequency compared to controls. The mEJP amplitude is increased, similar to that seen in Blos1^EY06269/+ mutant flies.

(Mullin et al., 2015)

The potentiation of baseline release that is seen in larvae expressing Dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 is no longer seen if the animals are also expressing P{GD12574} under the simultaneous control of both Scer\GAL4^elav-C155 and Scer\GAL4^sca.PC.

(Dickman et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^Mhc.PW/Dysb^UAS.cDa fails to rescue Dysb^e01028

(Dickman and Davis, 2009)

Comments

Presynaptic expression of Dysb^Scer\UAS.cDa using Scer\GAL4^elav-C155 restores the homeostatic quantal content increase to wild-type levels in Dysb^e01028 mutants.

(Gokhale et al., 2015)

Expression of dysb^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155, but not under the control of Scer\GAL4^Mhc.PW, rescues homeostatic compensation after treatment of neuromuscular junctions with with philanthotoxin-433 in dysb^e01028 mutants.

(Dickman and Davis, 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dysb^Scer\UAS.cDa

Dysb^UAS.cDa

dysb^Scer\UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Dickman

Secondary FlyBase IDs

References (4)

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