FlyBase Allele Report: Dmel\ninaE[G69D.GMR]

General Information

Symbol

Dmel\ninaE^G69D.GMR

Species

D. melanogaster

Name

FlyBase ID

FBal0241805

Feature type

allele

Associated gene

Dmel\ninaE

Associated Insertion(s)

Carried in Construct

P{GMR-ninaE.G69D}

Key Links

Transgenic product class

missense_variant

(Kang and Ryoo, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kang and Ryoo, 2009)

Mutagen

in vitro construct

(Kang and Ryoo, 2009)

Progenitor genotype

Carried in construct

P{GMR-ninaE.G69D}

Cytology

Description

GMR regulatory sequences drive expression of a mutated form of ninaE.

(Kang and Ryoo, 2009)

Amino acid replacement: G69D.

(Kang and Ryoo, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

ninaE

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of retinitis pigmentosa

CEA

(Huang et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of retinitis pigmentosa

is ameliorated by sip3^UAS.Tag:MYC

(Huang et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Morishita et al., 2013)

visible | adult stage

(Neves et al., 2016)

Phenotype Manifest In

eye

(Morishita et al., 2013)

eye, with Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

eye | adult stage

(Neves et al., 2016)

ommatidium

(Morishita et al., 2013)

Detailed Description

Statement

Reference

The expression of ninaE^G69D.GMR under the combined control of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU leads to abnormal eye morphology, as compared to controls.

(Huang et al., 2018)

Adult flies expressing ninaE^G69D.GMR show retinal degeneration phenotype.

(Neves et al., 2016)

ninaE^G69D.GMR mutants exhibit massive levels of cell death during development, resulting in adults eclosing with partially ablated eyes. These eyes display a glassy surface due to the ablation of the ommatidia.

(Morishita et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, suppressible by sip3^UAS.Tag:MYC/Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

ninaE^G69D.GMR has eye phenotype, suppressible by Cdk7^S164A.T170A

(Morishita et al., 2013)

ninaE^G69D.GMR has ommatidium phenotype, suppressible by Cdk7^S164A.T170A

(Morishita et al., 2013)

ninaE^G69D.GMR has eye phenotype, suppressible | partially by Mat1^KK107493/Scer\GAL4^GMR.PF

(Morishita et al., 2013)

ninaE^G69D.GMR has ommatidium phenotype, suppressible | partially by Mat1^KK107493/Scer\GAL4^GMR.PF

(Morishita et al., 2013)

ninaE^G69D.GMR has eye phenotype, suppressible by Scer\GAL4^GMR.PF/Cdk7^GD4167

(Morishita et al., 2013)

ninaE^G69D.GMR has ommatidium phenotype, suppressible by Scer\GAL4^GMR.PF/Cdk7^GD4167

(Morishita et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by sip3^GD3145/sip3^UAS.Tag:MYC/Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by sip3^GD3145/EMC10^KK104001, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by Pngl^KK101641/sip3^GD3145, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by Ube4A^KK101530/sip3^GD3145/Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by asrij^KK108914/sip3^GD3145/Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by sip3^GD3145/CG2126^GD3969, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by hiro^KK101387/sip3^GD3145/Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU, ninaE^G69D.GMR has eye phenotype, non-suppressible by CG6766^GD5521/sip3^GD3145, Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU

(Huang et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormal eye morphology resulting from the expression of ninaE^G69D.GMR under the combined control of Scer\GAL4^GMR.PU, Scer\GAL4^ey.PU is suppressed by the co-expression of sip3^{Scer\UAS.T:Hsap\MYC} alone, but not by co-expression of sip3^{Scer\UAS.T:Hsap\MYC} in combination with sip3^GD3145, CG32441^KK104001, Pngl^KK101641, CG11070^KK101530, asrij^KK108914, CG2126^GD3969, hiro^KK101387 or CG6766^GD5521 (in combination with Dicer-2, for efficient RNAi).

(Huang et al., 2018)

Expression of Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ reduces the severity of the retinal degeneration phenotype characteristic for adult flies expressing ninaE^G69D.GMR.

(Neves et al., 2016)

Knockdown of Mat1, through expression of Mat1^KK107493 under the control of Scer\GAL4^GMR.PF partially suppresses the eye size and ommatidial disarray defects found in ninaE^G69D.GMR mutants.

(Morishita et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99696

w^*; CyO/wg^Sp-1; P{GMR-ninaE.G69D}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rh1^G69D

(Morishita et al., 2013)

Rhodopsin-1^G69D

(Ryoo et al., 2013)

ninaE^G69D.GMR

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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