FlyBase Allele Report: Dmel\tutl[ex383]

General Information

Symbol

Dmel\tutl^ex383

Species

D. melanogaster

Name

FlyBase ID

FBal0241902

Feature type

allele

Associated gene

Dmel\tutl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Al-Anzi and Wyman, 2009)

Progenitor genotype

Cytology

Description

Imprecise excision of P{lacW}tutl^k14703 results in a 11,430bp deficiency within the tutl gene, disrupting all tutl transcripts.

(Al-Anzi and Wyman, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Al-Anzi and Wyman, 2009)

abnormal neuroanatomy (with Df(2L)ed-dp)

(Al-Anzi and Wyman, 2009)

abnormal neuroanatomy (with tutl⁰¹⁰⁸⁵)

(Al-Anzi and Wyman, 2009)

lethal - all die during embryonic stage

(Al-Anzi and Wyman, 2009)

some die during embryonic stage

(Al-Anzi and Wyman, 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Approximately 10% of tutl^ex383 embryonic segments have gaps in the longitudinal connectives and commissures. A minor enhancement of this phenotype is observed in embryos that are trans-heterozygous tutl^ex383/Df(2L)ed-dp.

Sixty-percent of tutl^ex383 homozygous embryos exhibit axon defasciculation with some axons projecting to the midline. Fas2-positive axon tracts maintain their relative distance from the midline, but axons emanating from all three fascicles bundle together as they cross the midline.

Embryos trans-heterozygous for tutl^ex383 and tutl⁰¹⁰⁸⁵ have defects in commissures, longitudinal connective, and Fas2-positive axon tracts that maintain their relative distance from the midline, but that have axons emanating from all three fascicles bundle together as they cross the midline.

In tutl^ex383 embryos, the ISNb motor axons succesfully reach the vicinity of their respective targets. However, the majority of them fail to send one or more of the final axon branches to contact their muscle targets. Half of the hemisegments also lack ISNd nerves.

Embryos trans-heterozygous for tutl^ex383 and tutl⁰¹⁰⁸⁵ have a similar embryonic motor axon projection defects to tutl^ex383 homozygotes, but with lower frequency.

(Al-Anzi and Wyman, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tutl[+]/tutl^ex383 is an enhancer of abnormal neuroanatomy phenotype of fra^GA957

(Al-Anzi and Wyman, 2009)

NOT Enhancer of

Statement

Reference

tutl[+]/tutl^ex383 is a non-enhancer of abnormal neuroanatomy phenotype of robo[+]/robo1⁵, sli¹

(Al-Anzi and Wyman, 2009)

tutl[+]/tutl^ex383 is a non-enhancer of abnormal neuroanatomy phenotype of robo1⁵, sli[+]/sli¹

(Al-Anzi and Wyman, 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

tutl[+]/tutl^ex383 is an enhancer of symmetrical commissure phenotype of fra^GA957

(Al-Anzi and Wyman, 2009)

tutl[+]/tutl^ex383 is an enhancer of larval longitudinal connective phenotype of fra^GA957

(Al-Anzi and Wyman, 2009)

NOT Enhancer of

Statement

Reference

tutl[+]/tutl^ex383 is a non-enhancer of commissure phenotype of robo[+]/robo1⁵, sli¹

(Al-Anzi and Wyman, 2009)

tutl[+]/tutl^ex383 is a non-enhancer of commissure phenotype of robo1⁵, sli[+]/sli¹

(Al-Anzi and Wyman, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

A tutl^ex383 heterozygous background dramatically enhances the fra^GA957 mutant phenotype. fra^GA957, tutl^ex383 double mutants typically have only one thin and fragmented commissure along the entire length of the embryo, indicating an extreme reduction in commissure formation and fragmented longitudinal connectives.

A tutl^ex383 heterozygous background dramatically enhances the Df(1)NP5 mutant phenotype. Df(1)NP5 ; tutl^ex383 double mutants typically have only one thin and fragmented commissure along the entire length of the embryo, indicating an extreme reduction in commissure formation and fragmented longitudinal connectives.

Triple trans-heterozygous sli¹/+, robo⁵/+, tutl^ex383/+ do not appear different from double sli¹/+, robo⁵/+ mutants, indicating that tutl does not function as an inhibitor of the sli pathway.

(Al-Anzi and Wyman, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tutl^ex383 is rescued by tutl^AT02763.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by tutl^GH15753.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by Scer\GAL4^sim.PS/tutl^HL01565.UAS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^HL01565.UAS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by tutl^LD28224.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by tutl^LD28224.UAS/Scer\GAL4^elav-C155

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by tutl^GH08133.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^GH08133.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by tutl^GH15753.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^GH15753.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by tutl^AT02763.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^AT02763.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by Scer\GAL4^sim.PS/tutl^HL01565.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^HL01565.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by tutl^LD28224.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by tutl^LD28224.UAS/Scer\GAL4^elav-C155

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by tutl^GH08133.UAS/Scer\GAL4^sim.PS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is rescued by Scer\GAL4^elav-C155/tutl^GH08133.UAS

(Al-Anzi and Wyman, 2009)

Not rescued by

tutl^ex383 is not rescued by tutl^GH15753.UAS/Scer\GAL4^repo

(Al-Anzi and Wyman, 2009)

tutl^ex383 is not rescued by tutl^AT02763.UAS/Scer\GAL4^repo

(Al-Anzi and Wyman, 2009)

tutl^ex383 is not rescued by Scer\GAL4^repo/tutl^HL01565.UAS

(Al-Anzi and Wyman, 2009)

tutl^ex383 is not rescued by tutl^LD28224.UAS/Scer\GAL4^repo

(Al-Anzi and Wyman, 2009)

tutl^ex383 is not rescued by Scer\GAL4^repo/tutl^GH08133.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is not rescued by tutl^GH15753.UAS/Scer\GAL4^repo

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is not rescued by tutl^AT02763.UAS/Scer\GAL4^repo

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is not rescued by Scer\GAL4^repo/tutl^HL01565.UAS

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is not rescued by tutl^LD28224.UAS/Scer\GAL4^repo

(Al-Anzi and Wyman, 2009)

Df(2L)ed-dp/tutl^ex383 is not rescued by Scer\GAL4^repo/tutl^GH08133.UAS

(Al-Anzi and Wyman, 2009)

Comments

Post-mitotic neuronal expression of tutl^{HL01565.Scer\UAS}, tutl^{GH15753.Scer\UAS}, tutl^{AT02763.Scer\UAS}, tutl^{LD28224.Scer\UAS} or tutl^{GH08133.Scer\UAS} under the control of either Scer\GAL4^sim.PS or Scer\GAL4^elav-C155 fully rescues the midline crossing defects found in tutl^ex383 and tutl^ex383/Df(2L)ed-dp mutants. Expression under the control of Scer\GAL4^repo does not rescue the phenotype, indicating that expression near the midline is important for rescue.

Post-mitotic neuronal expression of tutl^{AT02763.Scer\UAS} under the control of Scer\GAL4^elav-C155 is sufficient to rescue adult lethality found in tutl^ex383 mutants.

Expression of either tutl^{GH15753.Scer\UAS} or tutl^{AT02763.Scer\UAS} via Scer\GAL4^elav-C155 can reduce the motor axon projection defects found in tutl^ex383 homozygotes.

Expression of either tutl^{GH15753.Scer\UAS} or tutl^{AT02763.Scer\UAS} driven by the pan-neuronal driver Scer\GAL4^elav-C155 rescues most aspects of tutl^k14703/tutl^ex383 mutant eye defects.

(Al-Anzi and Wyman, 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tutl^ex383

(Sulkowski et al., 2011, Al-Anzi and Wyman, 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

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