FlyBase Allele Report: Dmel\flfl[N42]

General Information

Symbol

Dmel\flfl^N42

Species

D. melanogaster

Name

FlyBase ID

FBal0241927

Feature type

allele

Associated gene

Dmel\flfl

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Sousa-Nunes et al., 2009)

Progenitor genotype

flfl^EY03585

(Sousa-Nunes et al., 2009)

Cytology

Description

Imprecise excision of P{EPgy2}flfl^EY03585, located approximately 1kb upstream of the flfl translational start site generates a deletion extending into the flfl coding region, deleting the first 1075 base pairs of the coding sequence.

(Sousa-Nunes et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:13,686,811..13,688,938 [+]

Comment:

Deletion caused by imprecise excision of P{EPgy2}flfl^EY03585 extending from the insertion site and removing 1075 bases of the flfl coding sequence.

(Sousa-Nunes et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number (with Df(3R)Exel6170)

(Sousa-Nunes et al., 2009)

decreased occurrence of cell division | larval stage

(Connell et al., 2024, Sousa-Nunes et al., 2009)

lethal - all die before end of larval stage

(Sousa-Nunes et al., 2009)

some die during larval stage

(Sousa-Nunes et al., 2009)

Phenotype Manifest In

larval brain | larval stage

(Connell et al., 2024)

neuroblast (with Df(3R)Exel6170)

(Sousa-Nunes et al., 2009)

Detailed Description

Statement

Reference

Compared with wild-type, BrdU incorporation in flfl^N42 hemizygous brains is drastically reduced at 96 hours after larval hatching. In comparison with wild-type larvae, whch pupate between 96 an 120 hours after larval hatching and have ~120 central brain neuroblasts per brain lobe at 96 hours, flfl^N42 larvae fail to pupate, but can survive for up to 8 hours after larval hatching. Even after their extended larval phase, the number of central brain neuroblasts per brain lobe of flfl^N42 larvae only reaches approximately 80 and analysis of the mushroom body lineage progeny size reveals a cell-autonomous contribution of flfl towards proliferation. flfl^N42 larval brains have a higher proportion of neuroblasts that are phospho-histone-H3 positive, of which the majority show nuclear enrichment of mira and pros, suggesting the reduced proliferation could result from a block or delay at prophase.

(Sousa-Nunes et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions