FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\pcmhs.PG
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General Information
Symbol
Dmel\pcmhs.PG
Species
D. melanogaster
Name
heat shock construct of Grima
FlyBase ID
FBal0242033
Feature type
allele
Associated gene
Dmel\pcm
Associated Insertion(s)
Carried in Construct
P{hs-pcm.G}
Also Known As
hs-pcm
Key Links
Transgenic product class
cDNA
(Grima et al., 2008)
wild_type
(Grima et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Grima et al., 2008)
wild_type
(Grima et al., 2008)
Mutagen
in vitro construct
(Grima et al., 2008)
Progenitor genotype
Carried in construct
P{hs-pcm.G}
Cytology
Description

The Hsp70Bb promoter drives expression of a full length pcm cDNA (the 5.4 kb pcm open reading frame, plus 5' and 3' UTRs).

(Grima et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
pcm
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The heat-shock-induced expression of pcmhs.PG does not result in ectopic sex comb teeth in males, as compared to controls.

      (Nishioka et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      pcm[+]/pcmhs.PG is an enhancer of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      pcmhs.PG, mbf1[+], mbf12, pcmΔ1/pcmΔ1 is an enhancer of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      pcmhs.PG, mbf1[+], mbf12, pcmΔ1/pcmΔ1 is an enhancer of sex comb tooth | ectopic phenotype of Pc6

      (Nishioka et al., 2018)
      NOT Suppressor of
      Statement
      Reference

      pcmhs.PG, pcmΔ1/pcmΔ1 is a non-suppressor of sex comb tooth | ectopic phenotype of Pc6

      (Nishioka et al., 2018)

      pcmhs.PG, pcmΔ1/pcmΔ1 is a non-suppressor of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)

      pcmhs.PG, pcm5/pcm5 is a non-suppressor of sex comb tooth | ectopic phenotype of Psc1

      (Nishioka et al., 2018)
      Other
      Statement
      Reference

      mbf12, pcm[+]/pcmhs.PG has sex comb tooth | ectopic phenotype

      (Nishioka et al., 2018)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The heat-shock-induced expression of pcmhs.PG in mbf12 heterozygotes leads to a low penetrance ectopic sex comb teeth phenotype, as compared to controls.

      The ectopic sex comb teeth phenotype of Psc1 heterozygous males is partially suppressed by homozygosity for either pcmΔ1 or pcm5 and is exacerbated by the heat-shock-induced expression of pcmhs.PG; the phenotype suppression by either pcmΔ1 or pcm5 homozygosity is annulled by the heat-shock-induced expression of pcmhs.PG. The ectopic sex comb teeth phenotype of Psc1 heterozygous males is also exacerbated by the triple combinations of heterozygosity for mbf12, homozygosity for either pcmΔ1 or pcm5 and heat-shock-induced expression of pcmhs.PG.

      Similarly, the ectopic sex comb teeth phenotype of Pc6 heterozygous males is partially suppressed by homozygosity for pcmΔ1; this phenotype suppression is annulled either by the heat-shock-induced expression of pcmhs.PG. The ectopic sex comb teeth phenotype of Pc6 heterozygous males is also exacerbated by the triple combination of mbf12 heterozygosity, pcmΔ1 homozygosity and heat-shock-induced expression of pcmhs.PG.

      (Nishioka et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      pcmhs.PG rescues pcm5

      (Grima et al., 2008)

      pcmhs.PG rescues pcm5/Df(1)JA27

      (Grima et al., 2008)
      Comments

      Low levels of ectopic pcmhs.PG/+ rescue viability of pcm5/Df(1)JA27 at 19[o]C.

      The dull-wing phenotype in pcm5/Y males is rescued by a wildtype copy of pcmhs.PG.

      The crooked-leg phenotype in pcm5 adults is rescued by a wildtype copy of pcmhs.PG.

      The cleft thorax phenotype in pcm5 animals is rescued by a wildtype copy of pcmhs.PG.

      (Grima et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Expresion of pcm transgene is leaky, with ubiquitous expression and low levels throughout the life cycle at 19[o]C.

      (Grima et al., 2008)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pcmhs.PG
      Name Synonyms
      heat shock construct of Grima
      Secondary FlyBase IDs
        References (2)