FlyBase Allele Report: Dmel\His3.3A[2X1]

General Information

Symbol

Dmel\His3.3A^2X1

Species

D. melanogaster

Name

FlyBase ID

FBal0242706

Feature type

allele

Associated gene

Dmel\His3.3A

Associated Insertion(s)

Carried in Construct

Also Known As

H3.3A^2x1

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Sakai et al., 2009)

Progenitor genotype

P{SUPor-P}His3.3A^KG02455

(Sakai et al., 2009)

Cytology

Description

Imprecise excision of P{SUPor-P}His3.3A^KG02455, resulting in an approximately 400-bp deletion removing the entire coding sequence of His3.3A.

(Sakai et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromatin (with Df(2L)BSC110)

(Penke et al., 2018)

chromosome, telomeric region (with Df(2L)BSC110)

(Penke et al., 2018)

polytene chromosome (with Df(2L)BSC110)

(Penke et al., 2018)

polytene chromosome chromocenter (with Df(2L)BSC110)

(Penke et al., 2018)

wing disc (with Df(2L)BSC110)

(Penke et al., 2018)

Detailed Description

Statement

Reference

His3.3A^2X1/Df(2L)BSC110 transheterozygotes have an increased proportion of disorganized chromocenters in polytene chromosomes compared to controls.

Chromosomes in wing discs of His3.3A^2X1/Df(2L)BSC110 transheterozygotes have a disrupted chromatin architecture.

(Penke et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has sterile phenotype, suppressible by His3.3B^+t5

(Penke et al., 2018)

Other

Statement

Reference

His3.3A[+]/His3.3A^2X1, His3.3B^K9R has viable phenotype

(Penke et al., 2018)

His3.3A[+]/His3.3A^2X1, His3.3B^K9R has fertile phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has viable phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has sterile phenotype

(Penke et al., 2018)

Df(2L)His^C, His3.3A^2X1, His3.3B^K9R, His3^HisTA.wt/His3[+] has partially lethal - majority die phenotype

(Penke et al., 2018)

Df(2L)His^C, His3.3A^2X1, His3.3B^K9R, His3^HisTA.H3K9R has lethal - all die before end of larval stage phenotype

(Penke et al., 2018)

His3.3A^2X1, His3.3B⁰ has partially lethal - majority die phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K9R.Tag:FLAG, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^{S31A.Tag:FLAG}, His3.3B⁰ has viable phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^{S31A.Tag:FLAG}, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3B⁰ has sterile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^Tag:FLAG.cSa, His3.3B⁰ has viable phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^Tag:FLAG.cSa, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^H3.Tag:FLAG, His3.3B⁰ has viable phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^H3.Tag:FLAG, His3.3B⁰ has male sterile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K4R.Tag:FLAG, His3.3B⁰ has partially lethal - majority die phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K4R.Tag:FLAG, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K9R.Tag:FLAG, His3.3B⁰ has partially lethal - majority die phenotype

(Sakai et al., 2009)

Phenotype Manifest In

Other

Statement

Reference

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has wing disc phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has chromatin phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has chromosome, telomeric region phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has polytene chromosome phenotype

(Penke et al., 2018)

His3.3A^2X1/Df(2L)BSC110, His3.3B^K9R has polytene chromosome chromocenter phenotype

(Penke et al., 2018)

His3.3A^2X1, His3.3B⁰ has spermatid phenotype

(Sakai et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The sterility of His3.3B^K9R/His3.3B^K9R; His3.3A^2X1/Df(2L)BSC110 males and females is rescued by His3.3B^+t5.

(Penke et al., 2018)

His3.3A^2X1, His3.3B⁰ double mutants have reduced viability and are invariably sterile in both males and females.

Round developing spermatids are present in testes of His3.3A^2X1, His3.3B⁰ double mutant males but vary in size. Late elongating nuclei are never observed in the double mutants. Clear defects in meiosis are apparent by metaphase I, with diffuse chromosomes that are only partially condensed. Metaphase I with incompletely condensed chromosomes is followed by an aberrant anaphase in the double mutants. Further meiotic aberrations occur in anaphase II in His3.3A^2X1, His3.3B⁰ double mutant testes, such as chromosome bridges between sister chromatids. Compared to wild-types, His3.3A^2X1, His3.3B⁰ double mutant testes contain postmeiotic nuclei that are abnormal in size. The postmeiotic nuclear body is often fragmented into two-fifteen smaller bodies in the double mutants.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{T:Zzzz\FLAG.cSa} transgene are viable and fertile. The His3.3A^{T:Zzzz\FLAG.cSa} rescue construct restores all aspects of normal germ lines (meiotic segregation, the single nuclear body in postmeiotic nuclei, production of motile sperm and male fertility) to double mutant males.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{H3.T:Zzzz\FLAG} transgene are viable, but sterile. Meiotic defects and fragmentation of the postmeiotic nuclear body persists in His3.3A^2X1, His3.3B⁰ double mutant males carrying His3.3A^{H3.T:Zzzz\FLAG}.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{K4R.T:Zzzz\FLAG} transgene are fertile, but they show reduced viability. The His3.3A^2X1, His3.3B⁰ double mutant germ line cells also carrying His3.3A^{K4R.T:Zzzz\FLAG} show no defects.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{K9R.T:Zzzz\FLAG} transgene are fertile, but they show reduced viability.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{S31A.T:Zzzz\FLAG} transgene are viable and fertile.

(Sakai et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

68240

y¹ w^* His3.3B⁰; His3.3A^2X1/CyO, S²

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

H3.3A^2x1

(McPherson et al., 2023, Salzler et al., 2023, Leatham-Jensen et al., 2019, Armstrong et al., 2018, Penke et al., 2018)

H3.3A^null

(Salzler et al., 2023)

His3.3A^2X1

(Schneiderman et al., 2012, Sakai et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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