FlyBase Allele Report: Dmel\Gr33a[1]

General Information

Symbol

Dmel\Gr33a¹

Species

D. melanogaster

Name

FlyBase ID

FBal0243783

Feature type

allele

Associated gene

Dmel\Gr33a

Associated Insertion(s)

TI{TI}Gr33a¹

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Moon et al., 2009)

I-SceI

(Moon et al., 2009)

FLPase

(Moon et al., 2009)

ends-out gene targeting

(Moon et al., 2009, Moon et al., 2009)

Progenitor genotype

Associated Insertion(s)

TI{TI}Gr33a¹

Cytology

Description

The 5' part of the Gr33a locus has been replaced by a w^+mW.hs marker.

(Moon et al., 2009)

Ends-out homologous recombination at the Gr33a locus has resulted in the deletion of residues 1 to 199 of Gr33a.

(Moon et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

labellar taste bristle

(Moon et al., 2009, Moon et al., 2009)

short labellar taste bristle

(Sung et al., 2017)

Detailed Description

Statement

Reference

Gr33a¹ mutant flies do not show significant changes in the expected avoidance response to food laced with acetic acid (5%), as compared to controls.

(Rimal et al., 2019)

Gr33a¹ severely reduces the S-b sensilla responsiveness to the bitter chemicals LOB, BER, DEN normally observed in controls.

(Sung et al., 2017)

Gr33a¹/Gr33a¹ and Gr33a¹/Gr33a^GAL4 mutant males show no difference in courtship intensity as compared with wild type, but lose preference for younger females, instead showing no significant preference for either younger or older females.

(Hu et al., 2015)

Gr33a¹ mutant flies do not exhibit any defects in response to strychnine.

(Lee et al., 2015)

Gr33a¹ mutant adults display impaired avoidance of umbelliferone in a two-way choice feeding assay compared to wild-type as well as reduced frequency of action potentials invoked upon exposure to umbelliferone (measured on S3, S5, S6 and S9 sensillae).

Gr33a¹ mutant females display impaired umbelliferone as well as lobeline and quinine avoidance in an assay testing site selection for egg laying. However similarly to wild-type controls, they avoid lobeline and umbelliferone containing substrate without sucrose in favor of high sucrose-content substrate.

Gr33a¹ mutant larvae, like the wild-type, show preference for sucrose containing media over agarose-only in a binary choice larval behavior assay but unlike the wild-type keep preferring the sucrose media even if contaminated with umbelliferone.

(Poudel et al., 2015)

Gr33a¹ does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Homozygous Gr33a¹ mutant larvae do not have altered preference for larval-treated substrate, compared to controls.

(Mast et al., 2014)

Mutant flies show impaired avoidance to each of the bitter tastants papaverine and berberine in a two-way choice taste assay.

(Jeong et al., 2013)

The gustatory aversion of mutant flies to 6mM camphor in a two way-choice test is not significantly different from that seen in wild-type flies.

(Zhang et al., 2013)

Mutant flies show normal responses to both low salt (50mM NaCl) and high salt (500mM NaCl) in a two-way choice test.

(Zhang et al., 2013)

Mutant labellar taste bristles show a normal electrophysiological response to 50mM sucrose.

Mutant flies show a normal behavioural response to sucrose in a two-way choice test (preferring 5mM sucrose to 1mM sucrose).

Mutant labellar taste bristles show an impaired electrophysiological response to a number of bitter tasting compounds (caffeine, quinine, denatonium, berberine, lobeline, papaverine and strychnine).

(Moon et al., 2009)

Mutant labellar taste bristles show an impaired electrophysiological response to a number of bitter tasting compounds (caffeine, quinine, denatonium, berberine, lobeline, papaverine and strychnine).

Mutant flies show reduced avoidance to a number of bitter compounds (caffeine, quinine, denatonium, berberine, lobeline and papaverine) in a two-way choice assay compared to control flies.

Gr33a¹/Gr33a^GAL4 males show increased courtship towards passive, decapitated wild-type males compared to the behaviour of control males in this assay. However, courtship of the mutant males to decapitated or normal females is not significantly different from that of wild type.

Wild-type males show a normal average time to mating when mated with Gr33a¹/Gr33a^GAL4 females.

(Moon et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gr33a¹/Gr33a^GAL4 is rescued by Scer\GAL4^Gr33a-GAL4/Gr33a^UAS.cMa

(Hu et al., 2015)

Gr33a¹/Gr33a^GAL4 is rescued by Scer\GAL4^Gr33a-GAL4/Gr33a^UAS.cMa

(Moon et al., 2009)

Gr33a¹/Gr33a^GAL4 is rescued by Scer\GAL4^Gr33a-GAL4/Gr33a^UAS.cMa

(Moon et al., 2009)

Partially rescued by

Gr33a¹/Gr33a[+] is partially rescued by Scer\GAL4^Gr33a-GAL4/Gr33a^UAS.cMa

(Poudel et al., 2015)

Comments

Expression of Gr33a^Scer\UAS.cMa under the control of Scer\GAL4^Gr33a-GAL4 rescues the male courtship defect of Gr33a¹/Gr33a^GAL4 mutants.

(Hu et al., 2015)

Expression of Gr33a^Scer\UAS.cMa under the control of Scer\GAL4^Gr33a-GAL4 in the Gr33a¹ heterozygote adults significantly recovers the perturbed avoidance of umbelliferone in a two-way choice feeding assay as well as the site selection for oviposition assay and restores the reduction in frequency of action potentials invoked in the S6 sensilla upon umbelliferone exposure.

(Poudel et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

31427

w^*; TI{TI}Gr33a¹

31424

w^*; TI{TI}Gr33a¹; P{UAS-Gr33a.M}3

Notes on Origin

Discoverer