FlyBase Allele Report: Dmel\Ube3a[15B]

General Information

Symbol

Dmel\Ube3a^15B

Species

D. melanogaster

Name

FlyBase ID

FBal0244597

Feature type

allele

Associated gene

Dmel\Ube3a

Associated Insertion(s)

Carried in Construct

Also Known As

dube3a^15B, 15B

Key Links

Allele class

loss of function allele

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lee et al., 2014)

loss of function allele

(Wu et al., 2008)

Mutagen

Delta2-3 transposase

(Wu et al., 2008)

Progenitor genotype

P{EP}Ube3a^EP3214

(Wu et al., 2008)

Cytology

Description

Imprecise excision of the progenitor insertion resulting in a 1.2kb deletion in Ube3a. The transcription start site is retained.

(Wu et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Angelman syndrome

CEA

(Valdez et al., 2015, Wu et al., 2008)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Valdez et al., 2015)

NMJ bouton | increased number | third instar larval stage (with Df(3L)ED4470)

(Li et al., 2016)

NMJ bouton | increased number | third instar larval stage (with Ube3a³⁵)

(Li et al., 2016)

Detailed Description

Statement

Reference

Ube3a^15B/Ube3a^15B third instar larvae have similar numbers of total boutons (and satellite boutons) at the neuromuscular junction compared to wild type; Ube3a³⁵/Ube3a^15B or Ube3a^15B/Df(3L)ED4470 third instar larvae have a significant increase in total boutons (and satellite boutons) at the neuromuscular junction (compared to Ube3a^15B/Ube3a^15B).

(Li et al., 2016)

Ube3a^15B/Ube3a^15B larvae show a decrease (significantly more than wild type) in EJP amplitude during rapid stimulation (30 stimulations at 15Hz) and have significantly more negative muscle resting membrane potential compared to wild type. There is no significant difference in amplitude of the first EJP between Ube3a^15B/Ube3a^15B and wild type, though short-term facilitation is seen significantly more in wild type larvae. There is no significant difference in active zone density, bouton numbers or synaptic vesicle number or size at the neuromuscular junction of Ube3a^15B/Ube3a^15B third instar larvae compared to wild type.

(Valdez et al., 2015)

Ube3a^15B/+ flies have normal eyes.

(Kim et al., 2013)

Mutant flies do not show taste adaptation in response to a camphor diet, in contrast to wild-type flies, which show reduced camphor avoidance and reduced camphor-induced action potentials in the S6 taste sensilla after being maintained on a camphor diet.

(Zhang et al., 2013)

Ube3a^15B flies, which exhibit a reduction in dopamine, also exhibit a concurrent reduction in locomotor activity.

(Ferdousy et al., 2011)

Both young and old Ube3a^15B mutant flies perform significantly more poorly than age-matched wild-type flies in climbing assays. Mutant flies show no obvious defects in their ability to initiate flight.

Ube3a^15B mutant flies show defective 1-day memory compared to wild-type after a period of spaced-training sessions in olfactory learning and memory assays. Levels of memory are similar compared to wild-type animals after a period of massed-training sessions, or after a single training session. Mutant and wild-type flies perform similarly in direct tests of shock reactivity and olfaction.

Young Ube3a^15B mutant males and old mutant flies of each sex, entrained to a 12h light/dark cycle for 3 days, all display arrhythmic rest/activity behaviour cycles.

Ube3a^15B mutant flies exposed to mechanical or thermal stress do not show any sign of paralysis.

(Wu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ube3a[+]/Ube3a^15B is a suppressor | partially of visible phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Kim et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Ube3a[+]/Ube3a^15B is a suppressor | partially of eye phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Kim et al., 2013)

Ube3a[+]/Ube3a^15B is a suppressor | partially of ommatidium phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Kim et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Ube3a^15B/+ suppresses rough eye phenotypes with ommatidial disorganization in flies with expression of Hsap\MECP2^Scer\UAS.FL driven by Scer\GAL4^GMR.PU.

(Kim et al., 2013)

Complementation and Rescue Data

Rescued by

Ube3a^15B is rescued by Ube3a^Tag:MYC

(Zhang et al., 2013)

Ube3a^15B is rescued by Ube3a^+t13

(Wu et al., 2008)

Not rescued by

Ube3a^15B is not rescued by Ube3a^tC941A

(Wu et al., 2008)

Comments

Ube3a^+t13 restores wild-type climbing ability in Ube3a^15B mutant flies.

Ube3a^tC941A does not rescue the climbing-defects observed in Ube3a^15B mutant flies.

(Wu et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90380

w^*; Ube3a^15B/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

15B

(Lee et al., 2014)

15b

(Kim et al., 2013)

Dube3a^15b

(Valdez et al., 2015, Jensen et al., 2013)

Ube3a^15B

Ube3a^15b

(Ferdousy et al., 2011)

dube3a^15B

(Wu et al., 2008, Wu et al., 2008)

ube3a^15B

(Li et al., 2016)

ube3a^15b

(Zhang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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