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General Information
Symbol
Dmel\Ube3a15B
Species
D. melanogaster
Name
FlyBase ID
FBal0244597
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dube3a15B, 15B
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion resulting in a 1.2kb deletion in Ube3a. The transcription start site is retained.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ube3a15B/Ube3a15B third instar larvae have similar numbers of total boutons (and satellite boutons) at the neuromuscular junction compared to wild type; Ube3a35/Ube3a15B or Ube3a15B/Df(3L)ED4470 third instar larvae have a significant increase in total boutons (and satellite boutons) at the neuromuscular junction (compared to Ube3a15B/Ube3a15B).

Ube3a15B/Ube3a15B larvae show a decrease (significantly more than wild type) in EJP amplitude during rapid stimulation (30 stimulations at 15Hz) and have significantly more negative muscle resting membrane potential compared to wild type. There is no significant difference in amplitude of the first EJP between Ube3a15B/Ube3a15B and wild type, though short-term facilitation is seen significantly more in wild type larvae. There is no significant difference in active zone density, bouton numbers or synaptic vesicle number or size at the neuromuscular junction of Ube3a15B/Ube3a15B third instar larvae compared to wild type.

Ube3a15B/+ flies have normal eyes.

Mutant flies do not show taste adaptation in response to a camphor diet, in contrast to wild-type flies, which show reduced camphor avoidance and reduced camphor-induced action potentials in the S6 taste sensilla after being maintained on a camphor diet.

Ube3a15B flies, which exhibit a reduction in dopamine, also exhibit a concurrent reduction in locomotor activity.

Both young and old Ube3a15B mutant flies perform significantly more poorly than age-matched wild-type flies in climbing assays. Mutant flies show no obvious defects in their ability to initiate flight.

Ube3a15B mutant flies show defective 1-day memory compared to wild-type after a period of spaced-training sessions in olfactory learning and memory assays. Levels of memory are similar compared to wild-type animals after a period of massed-training sessions, or after a single training session. Mutant and wild-type flies perform similarly in direct tests of shock reactivity and olfaction.

Young Ube3a15B mutant males and old mutant flies of each sex, entrained to a 12h light/dark cycle for 3 days, all display arrhythmic rest/activity behaviour cycles.

Ube3a15B mutant flies exposed to mechanical or thermal stress do not show any sign of paralysis.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

Ube3a[+]/Ube3a15B is a suppressor | partially of visible phenotype of Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU

Phenotype Manifest In
Suppressor of
Statement
Reference

Ube3a[+]/Ube3a15B is a suppressor | partially of eye phenotype of Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU

Ube3a[+]/Ube3a15B is a suppressor | partially of ommatidium phenotype of Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Ube3a15B/+ suppresses rough eye phenotypes with ommatidial disorganization in flies with expression of Hsap\MECP2Scer\UAS.FL driven by Scer\GAL4GMR.PU.

Complementation and Rescue Data
Not rescued by
Comments

Ube3a+t13 restores wild-type climbing ability in Ube3a15B mutant flies.

Ube3atC941A does not rescue the climbing-defects observed in Ube3a15B mutant flies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)