FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Madm3T4
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General Information
Symbol
Dmel\Madm3T4
Species
D. melanogaster
Name
FlyBase ID
FBal0245106
Feature type
allele
Associated gene
Dmel\Madm
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Gluderer et al., 2010)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q46X.

(Gluderer et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:5,743,120..5,743,120 [+]
Nucleotide change:

C5743120T

Amino acid change:

Q46term | Madm-PA; Q46term | Madm-PB

Reported amino acid change:

Q46term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Gluderer et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Madm3T4/Madm3T4 mutant intestinal stem cell clones have significantly fewer cells and a smaller size than control clones.

      (Nie et al., 2015)

      Madm3T4 mutants exhibit a small head (pinhead) phenotype.

      Development of Madm3T4 mutant larvae ceases mostly in the third larval instar and in the pre-pupal stage. The onset of the pre-pupal stage is delayed by 2 to 10 days. A few adults are produced (less than 1% of the expected Mendelian ratio).

      The body size of the rare Madm3T4 mutant flies that survive to adulthood is reduced compared to controls, with females being almost 40% lighter than controls. Madm3T4 escapers also display malformations such as eye and wing defects, including rotation defects, missing and extra photoreceptors, fused ommatidia, and cell-fate transformations. Similar patterning defects are observed in Madm3T4 mutant eye clones. The wing phenotypes range from no defects to wing notches and an incomplete wing vein V.

      (Gluderer et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Madm3T4 is rescued by MadmUAS.cGa/Scer\GAL4arm.PS

      (Gluderer et al., 2010)

      Madm3T4 is rescued by Madm+t5.8

      (Gluderer et al., 2010)
      Comments

      Expression of Madm+t.2240 rescues all the phenotypes associated with Madm3T4 mutants.

      (Gluderer et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Based on the severity of the pinhead phenotype, when the mutant is over Df(3R)Exel7283, the following Madm alleles can be ranked from the most severe to the weakest: Madm2D2 = Madm2U3 = Madm3G5 > Madm3Y2 = Madm4S3 = Madm7L2 > Madm3T4.

      (Gluderer et al., 2010)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Madm3T4
      (Nie et al., 2015, Gluderer et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)