FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\BuffyRNAi.UAS
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General Information
Symbol
Dmel\BuffyRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0245185
Feature type
allele
Associated gene
Dmel\Buffy
Associated Insertion(s)
Carried in Construct
P{UAS-Buffy.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Brachmann, 2010.8.2)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Brachmann, 2010.8.2)
Mutagen
in vitro construct
(Brachmann, 2010.8.2)
Progenitor genotype
Carried in construct
P{UAS-Buffy.RNAi}
Cytology
Description

UASdrives expression of an RNAi construct targeting Buffy.

(Brachmann, 2010.8.2)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Buffy
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Parkinson's disease
      CEA
      (M'Angale and Staveley, 2016)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Parkinson's disease
      modeled by Hsc70-5KK100233
      (Zhu et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies with expression of BuffydsRNA.Scer\UAS driven by Scer\GAL4Ddc.PL have a decreased lifespan and impaired climbing ability compared to controls. Flies with expression of BuffydsRNA.Scer\UAS driven by Scer\GAL4GMR.PU have a rough eye phenotype.

      (M'Angale and Staveley, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BuffydsRNA.Scer\UAS enhances shortened lifespan in flies with expression of Hsap\SNCAScer\UAS.cFa driven by Scer\GAL4Ddc.PL. Co-expression of BuffydsRNA.Scer\UAS enhances the rough eye phenotype seen in flies with expression of Hsap\SNCAScer\UAS.cFa driven by Scer\GAL4GMR.PU.

      (M'Angale and Staveley, 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      BuffyRNAi.UAS
      BuffydsRNA.Scer\UAS
      BuffydsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (4)