FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Mdh21
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General Information
Symbol
Dmel\Mdh21
Species
D. melanogaster
Name
FlyBase ID
FBal0246393
Feature type
allele
Associated gene
Dmel\Mdh2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Wang et al., 2010)
Mutagen
ethyl methanesulfonate
(Wang et al., 2008)
Progenitor genotype
Cytology
Description

236bp deletion that shifts the reading frame after codon 242, changing amino acid residues 243-253 from GAGSATLSMAY to EGQEEHPEGHX.

(Wang et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:18,228,215..18,228,450 [+]
Comment:

Approximate boundaries of a 236 bp deletion that shift the reading frame after residue 242 leading to early translation termination afte 10 novel amino acids.

(Wang et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Most of the mid-third instar (wandering behaviour) and late larval (everting anterior spiracles, barrel shape) ecdysone-triggered responses are normal in Mdh21/Df(3R)Exel6178 animals. However, defects in midgut programmed cell death are seen. The prepupal ecdysone-triggered responses (head eversion, wing and leg inflation and leg extension) also occur normally. In the salivary gland, mid-third instar glue synthesis is normal, late larval glue secretion is normal, but prepupal salivary gland programmed cell death is defective.

      (Ihry and Bashirullah, 2014)

      46% of Mdh21/Mdh22 pupae at 24 hr APF show persistent larval salivary glands and none eclose.

      Mdh21/Df(3R)Exel6178 pupae appear virtually identical to controls at 12 hr APF, with no apparent defects in adult head eversion or leg eversion, and no delay between puparium formation and head eversion. However, whereas control salivary glands rupture and degrade soon after head eversion, mutant glands remain intact and maintain their morphological integrity. At 24 hr APF, 40-42% of Mdh21/Df(3R)Exel6178 pupae show persistent larval salivary glands and none eclose.

      Mdh21/Df(3R)Exel6178 salivary glands do not exhibit caspase-driven programmed cell death at 14 hr or 20 hr APF, in contrast to salivary glands from controls. However, salivary gland autophagy is induced normally.

      (Wang et al., 2010)

      Mutants show defects in destruction of the larval salivary glands, having persistant larval salivary glands at the pupal stage.

      (Wang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Mdh22/Mdh21 is rescued by Mdh2Tag:MYC

      (Wang et al., 2010)

      Mdh21/Df(3R)Exel6178 is rescued by Mdh2UAS.cWa

      (Wang et al., 2010)
      Comments

      One copy of Mdh2T:Hsap\MYC restores normal salivary gland cell death and efficiently rescues pupal lethality in Mdh21/Mdh22 animals.

      Scer\GAL4Act5C.PU-mediated expression of Mdh2Scer\UAS.cWa restores normal salivary gland cell death and efficiently rescues pupal lethality in Mdh21/Df(3R)Exel6178 animals.

      (Wang et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      14144
      (Wang et al., 2008)
      CG799814144
      (Wang et al., 2010)
      Mdh21
      (Wang et al., 2010)
      l(3)psg714144
      (Wang et al., 2008)
      mdh21
      (Ihry and Bashirullah, 2014)
      mdh2psg7b
      (Ihry and Bashirullah, 2014)
      psg7b
      (Ihry and Bashirullah, 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)