FlyBase Allele Report: Dmel\mute[1281]

General Information

Symbol

Dmel\mute¹²⁸¹

Species

D. melanogaster

Name

FlyBase ID

FBal0246406

Feature type

allele

Associated gene

Dmel\mute

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Bulchand et al., 2010)

Mutagen

ethyl methanesulfonate

(Bulchand et al., 2010)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C559T.

(Bulchand et al., 2010)

Amino acid replacement: Q187term.

(Bulchand et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:16,954,532..16,954,532 [-]

Nucleotide change:

C16954532T

Reported nucleotide change:

C559T

Amino acid change:

Q187term | mute-PC

Reported amino acid change:

Q187term

(Bulchand et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | germline clone | recessive

(Bulchand et al., 2010)

lethal (with Df(2R)ED2751)

(Kahsai et al., 2016)

lethal - all die during embryonic stage (with Df(2R)Exel6065)

(Bulchand et al., 2010)

some die during embryonic stage (with Df(2R)Exel6065)

(Bulchand et al., 2010)

Phenotype Manifest In

larval somatic muscle cell

(Bulchand et al., 2010)

larval somatic muscle cell (with Df(2R)Exel6065)

(Bulchand et al., 2010)

larval somatic muscle cell (with mute^NP1160)

(Bulchand et al., 2010)

Detailed Description

Statement

Reference

Females containing homozygous germline clones do not lay eggs.

Homozygous stage 16 embryos show a severe loss of muscle mass compared to controls.

mute¹²⁸¹/Df(2R)Exel6065 embryos show no significant difference in somatic muscle mass or morphology at stage 14 compared to control embryos. However, by stage 15, some muscles appear thinner than normal in the mutant embryos and some muscles are no longer detectable. At stage 16, many muscles appear significantly thinner than normal in the mutant embryos and some muscles are no longer detectable. This phenotype is not biased to any specific somatic muscle type and some of the muscles appear to detached at least at one end. By stage 17, the muscles have degenerated so that they have lost the characteristic wild-type morphology and are spherical in shape or are extremely thin.

(Bulchand et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

mute¹²⁸¹/Df(2R)Exel6065 has larval somatic muscle cell phenotype, non-suppressible by Df(3L)H99

(Bulchand et al., 2010)

Other

Statement

Reference

Slbp¹⁵, mute¹²⁸¹/mute[+] has larval somatic muscle cell phenotype

(Bulchand et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The thin muscles characteristic of late stage mute¹²⁸¹/Df(2R)Exel6065 embryos are still seen if they are also homozygous for Df(3L)H99.

More than half of mute¹²⁸¹/+ ; Slbp¹⁵/Slbp¹⁵ stage 16 embryos have somatic muscles that appear significantly thinner than normal. Some muscles are detached while others appear to be missing.

(Bulchand et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mute¹²⁸¹/Df(2R)Exel6065 is rescued by Scer\GAL4^how-24B/mute^L.UAS

(Bulchand et al., 2010)

mute¹²⁸¹/Df(2R)Exel6065 is rescued by Scer\GAL4^how-24B/mute^ΔC.L.UAS

(Bulchand et al., 2010)

Partially rescued by

mute¹²⁸¹/Df(2R)Exel6065 is partially rescued by Scer\GAL4^how-24B/mute^S.UAS

(Bulchand et al., 2010)

Not rescued by

mute¹²⁸¹/Df(2R)Exel6065 is not rescued by Scer\GAL4^how-24B/mute^{ΔC-Atro.L.UAS}

(Bulchand et al., 2010)

Comments

The muscle wasting phenotype of mute¹²⁸¹/Df(2R)Exel6065 embryos is completely rescued by expression of mute^L.Scer\UAS or mute^{ΔC.L.Scer\UAS} under the control of Scer\GAL4^how-24B.

The muscle wasting phenotype of mute¹²⁸¹/Df(2R)Exel6065 embryos is not rescued by expression of mute^{ΔC-Atro.L.Scer\UAS} under the control of Scer\GAL4^how-24B.

The muscle wasting phenotype of mute¹²⁸¹/Df(2R)Exel6065 embryos is partially rescued by expression of mute^S.Scer\UAS under the control of Scer\GAL4^how-24B.

(Bulchand et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34055

y¹ w^*; mute¹²⁸¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mute¹²⁸¹

(Bivik et al., 2015, Bulchand et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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