FlyBase Allele Report: Dmel\wg[S239A.UAS.Tag:HA]

General Information

Symbol

Dmel\wg^{S239A.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0246835

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.HA.S239A}

Key Links

Transgenic product class

missense_variant

(Franch-Marro et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Franch-Marro et al., 2008)

Mutagen

in vitro construct

(Franch-Marro et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-wg.HA.S239A}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form a wg carrying a S239 to A amino acid substitution and an Tag:HA tag.

(Franch-Marro et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^nub-AC-62

(Baena-Lopez et al., 2009)

hyperplasia | somatic clone, with Scer\GAL4^en-e16E

(Baena-Lopez et al., 2009)

hyperplasia | somatic clone, with Scer\GAL4^hh-Gal4

(Baena-Lopez et al., 2009)

visible, with Scer\GAL4^ap-md544

(Franch-Marro et al., 2008)

visible, with Scer\GAL4^en-e16E

(Baena-Lopez et al., 2009)

visible, with Scer\GAL4^hh-Gal4

(Baena-Lopez et al., 2009)

visible, with Scer\GAL4^nub-AC-62

(Baena-Lopez et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^ap-md544

(Franch-Marro et al., 2008)

wing blade, with Scer\GAL4^en-e16E

(Baena-Lopez et al., 2009)

wing blade, with Scer\GAL4^hh-Gal4

(Baena-Lopez et al., 2009)

wing blade, with Scer\GAL4^nub-AC-62

(Baena-Lopez et al., 2009)

Detailed Description

Statement

Reference

Expression of wg^{S239A.Scer\UAS.T:Ivir\HA1} via Scer\GAL4^nub-AC-62 causes substantial enlargement of the wing compared with controls.

Expression of wg^{S239A.Scer\UAS.T:Ivir\HA1} with either Scer\GAL4^hh-Gal4 or Scer\GAL4^en-e16E leads to marked increase in the size of the posterior compartment. Compared with controls, the size of the anterior compartment is also increased, although less markedly than that of the posterior compartment.

The surface area of the territory colonised by randomly marked clones in wing imaginal discs expressing wg^{S239A.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^hh-Gal4 is larger in the posterior compartment (where wg^{S239A.Scer\UAS.T:Ivir\HA1} is expressed) than in the anterior compartment. Cell-doubling time appears to be reduced by about 22% on average in the wg^{S239A.Scer\UAS.T:Ivir\HA1}-expressing posterior compartment compared to those in control anterior compartments.

(Baena-Lopez et al., 2009)

Expression of wg^{S239A.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^ap-md544 prevents complete fusion of the notum but has no effect on wing patterning (e.g. no ectopic bristles).

(Franch-Marro et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^hh-Gal4/wg^{S239A.UAS.Tag:HA} partially rescues wg^l-17

(Baena-Lopez et al., 2009)

Comments

Expression of wg^{S239A.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^hh-Gal4 in posterior wing compartments composed of homozygous wg^l-17 mutant cells substantially restores wing size and the rate of cell proliferation. Rescued wings appear well proportioned, but they lack a proper margin in the posterior compartment.

(Baena-Lopez et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

wg^{S239A.Scer\UAS.T:Ivir\HA1}

wg^{S239A.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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