FlyBase Allele Report: Hsap\MAPT[AP.UAS]

General Information

Symbol

Hsap\MAPT^AP.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0247002

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\MAPT.AP}

Also Known As

UAS-Tau^AP

Key Links

Transgenic product class

missense_variant

(Fulga et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Fulga et al., 2007)

Mutagen

in vitro construct

(Fulga et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Hsap\MAPT.AP}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\MAPT, carrying mutations in fourteen disease-associated Ser-Thr-Pro sites (Thr 111, Thr 153, Ser 175, Thr 181, Ser 199, Ser 202, Thr 205, Thr 212, Thr 217, Thr 231, Ser 235, Ser 396, Ser 404, and Ser 422) to alanine.

(Fulga et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Talmat-Amar et al., 2011, Yeh et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Fulga et al., 2007)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite | third instar larval stage, with Scer\GAL4^ppk.PG

(Yeh et al., 2010)

dopaminergic PPL1 neuron | progressive, with Scer\GAL4^ple.PF

(Wu et al., 2013)

eye, with Scer\GAL4^GMR.PF

(Fulga et al., 2007)

eye, with Scer\GAL4^GMR.PU

(Wu et al., 2013, Yeh et al., 2010)

larval abdominal segmental nerve | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Talmat-Amar et al., 2011)

macrochaeta, with Scer\GAL4^Eq

(Yeh et al., 2010)

sensory organ cell, with Scer\GAL4^Eq

(Yeh et al., 2010)

wing blade | adult stage, with Scer\GAL4^RapGAP1-OK6

(Talmat-Amar et al., 2011)

Detailed Description

Statement

Reference

Flies expressing Hsap\MAPT^AP.Scer\UAS under the control of Scer\GAL4^ple.PF show a significant loss of PPL1 dopaminergic neurons in the brain by 2 weeks after eclosion, with the phenotype becoming more severe as the flies age.

(Wu et al., 2013)

Overexpression of Hsap\MAPT^AP.UAS under the control of Scer\GAL4^RapGAP1-OK6 in larval segmental nerves shows no significant reduction in MT density; a significant decrease in the number of mobile vesicles with in axons; effects synaptic vesicle kinetics (there is a significant increase in synaptic vesicle pausing time, a significant decrease in net distance run per sec, and a significant decrease in anterograde vesicular transport) as compared to WT controls. Emerging flies have unexpanded wings.

(Talmat-Amar et al., 2011)

Expression of Hsap\MAPT^AP.Scer\UAS under the control of Scer\GAL4^GMR.PF is not associated with toxicity in Drosophila.

(Feuillette et al., 2010)

Compared to controls, expression of Hsap\MAPT^AP.Scer\UAS under the control of Scer\GAL4^elav.PU does not result in mushroom body abnormalities.

(Kosmidis et al., 2010)

Expression of Hsap\MAPT^AP.Scer\UAS in the developing eye under the control of Scer\GAL4^GMR.PF generates a mild rough eye phenotype.

Expression of Hsap\MAPT^AP.Scer\UAS in the developing external sensory organs under the control of Scer\GAL4^Eq1 generates a mild degree of bristle loss.

(Yeh et al., 2010)

Flies expressing Hsap\MAPT^AP.Scer\UAS under the control of Scer\GAL4^GMR.PF have normal eyes.

Expression of Scer\GAL4^ppk.PG>Hsap\MAPT^AP.Scer\UAS results in a severe reduction in ddaC dendritic tree complexity and density in larvae compared with wild-type.

(Yeh et al., 2010)

Expression of Hsap\MAPT^E14.Scer\UAS in the retina under the control of Scer\GAL4^GMR.PF results in a mild rough-eye phenotype.

(Fulga et al., 2007)

Expression of Hsap\MAPT^AP.Scer\UAS in the eye under the control of Scer\GAL4^GMR.PF has no significant toxicity.

(Steinhilb et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\MAPT^AP.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Act5C^UAS.GFP, Scer\GAL4^GMR.PF

(Fulga et al., 2007)

Hsap\MAPT^AP.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}, Scer\GAL4^GMR.PF

(Fulga et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Act5C^{Scer\UAS.T:Avic\GFP} in animals expressing Hsap\MAPT^AP.Scer\UAS in the retina under the control of Scer\GAL4^GMR.PF does not enhance the rough-eye phenotype.

Expression of Hsap\APP^{Aβ42.Scer\UAS} in flies expressing Hsap\MAPT^AP.Scer\UAS under the control of Scer\GAL4^GMR.PF does not enhance the rough-eye phenotype.

(Fulga et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\MAPT^AP.Scer\UAS

Hsap\MAPT^AP.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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