FlyBase Allele Report: Dmel\Gyc89Da[UAS.cVa]

General Information

Symbol

Dmel\Gyc89Da^UAS.cVa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Vermehren-Schmaedick

FlyBase ID

FBal0247416

Feature type

allele

Associated gene

Dmel\Gyc89Da

Associated Insertion(s)

Carried in Construct

P{UAS-Gyc89Da.V}

Also Known As

^UASGyc-89Da

Key Links

Transgenic product class

wild_type

(Vermehren-Schmaedick et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Vermehren-Schmaedick et al., 2010)

Mutagen

in vitro construct

(Vermehren-Schmaedick et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Gyc89Da.V}

Cytology

Description

UASt regulatory sequences drive expression of the Gyc89Da open reading frame.

(Vermehren-Schmaedick et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Gyc89Da

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Gyc89Db.3.4/Gyc89Da^UAS.cVa is a suppressor of abnormal taste perception phenotype of Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2011)

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 is a suppressor of abnormal stress response | third instar larval stage phenotype of Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2010)

Scer\GAL4^Gyc89Db.3.4/Gyc89Da^UAS.cVa is a suppressor of abnormal stress response | third instar larval stage phenotype of Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2010)

NOT Suppressor of

Statement

Reference

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 is a non-suppressor of abnormal taste perception phenotype of Gyc89Db^MB03197

(Vermehren-Schmaedick et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The defect in the hypoxia escape response that is seen in Gyc-89Db^MB03197 larvae is rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of either Scer\GAL4^Gyc-89Da.1.9 or Scer\GAL4^Gyc-89Db.3.4.

The failure to change larval exploratory behaviour that is seen in Gyc-89Db^MB03197 larvae downshifted from 21 to 19% oxygen or upshifted from 21 to 25% oxygen is rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4, but is not rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9.

(Vermehren-Schmaedick et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 rescues Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2011)

Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9 rescues Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2010)

Scer\GAL4^Gyc89Db.3.4/Gyc89Da^UAS.cVa rescues Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2010)

Fails to rescue

Scer\GAL4^Gyc89Db.3.4/Gyc89Da^UAS.cVa fails to rescue Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2011)

Comments

The lack of caffeine avoidance seen in Gyc-89Db^MB03197 or Gyc-89Db^MB03197/Gyc-89Da^e01821 double mutants can be rescued through expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9. In contrast, expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4 fails to suppress the sucrose ambivalence phenotype.

The loss of caffeine avoidance behaviour at low concentrations of caffeine in Gyc-89Da^e01821 mutants is rescued by expressing Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4.

(Vermehren-Schmaedick et al., 2011)

The defect in the hypoxia escape response that is seen in Gyc-89Da^e01821 larvae is rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of either Scer\GAL4^Gyc-89Da.1.9 or Scer\GAL4^Gyc-89Db.3.4.

The failure to change larval exploratory behaviour that is seen in Gyc-89Da^e01821 larvae downshifted from 21 to 15% oxygen is rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9, but is not rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4.

(Vermehren-Schmaedick et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93115

w^*; P{UAS-Gyc89Da.V}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

^UASGyc-89Da

(Vermehren-Schmaedick et al., 2010)

Gyc-89Da^Scer\UAS.cVa

Gyc89Da^Scer\UAS.cVa

Gyc89Da^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Vermehren-Schmaedick

Secondary FlyBase IDs

References (2)

Report Sections

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