FlyBase Allele Report: Dmel\par-1[RNAi.UAS.cZa]

General Information

Symbol

Dmel\par-1^RNAi.UAS.cZa

Species

D. melanogaster

Name

FlyBase ID

FBal0247448

Feature type

allele

Associated gene

Dmel\par-1

Associated Insertion(s)

Carried in Construct

P{UAS-par-1.RNAi.Z}

Also Known As

UAS-PAR-1 RNAi

Key Links

Transgenic product class

RNAi_reagent

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-par-1.RNAi.Z}

Cytology

Description

UAS regulatory sequences drive expression of a par RNAi construct containing genomic DNA-cDNA hybrid sequences.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

par-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Lee et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^Mhc.PW

(Zhang et al., 2007)

Phenotype Manifest In

centrosome | adult stage | male, with Scer\GAL4^{VP16.nanos.UTR}

(Yuan et al., 2012)

male germline stem cell, with Scer\GAL4^{VP16.nanos.UTR}

(Yuan et al., 2012)

mitotic spindle | adult stage | male, with Scer\GAL4^{VP16.nanos.UTR}

(Yuan et al., 2012)

NMJ bouton, with Scer\GAL4^Mhc.PW

(Zhang et al., 2007)

subsynaptic reticulum, with Scer\GAL4^Mhc.PW

(Zhang et al., 2007)

Detailed Description

Statement

Reference

Germline stem cells (GSCs) in newly eclosed males expressing par-1^{dsRNA.Scer\UAS.cZa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} show a high frequency of centrosome misorientation and frequently undergo mitosis with misoriented spindles (mitosis with misoriented spindles is not seen in wild-type male GSCs). The mitotic index of male GSCs expressing par-1^{dsRNA.Scer\UAS.cZa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is comparable to that of control GSCs.

(Yuan et al., 2012)

Some boutons at the neuromuscular junction of muscle 6/7 are smaller than normal and irregularly shaped in larvae expressing par-1^{dsRNA.Scer\UAS.cZa} under the control of Scer\GAL4^Mhc.PW. There is a mild increase in bouton number in these animals compared to controls. The subsynaptic reticulum is overgrown in the mutant boutons compared to controls.

Expression of par-1^{dsRNA.Scer\UAS.cZa} under the control of Scer\GAL4^elav.PU has no significant effect on the number of boutons at the neuromuscular junction of muscle 6/7.

(Zhang et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/par-1^RNAi.UAS.cZa is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^en-e16E, ft^UAS.cMa

(Harumoto et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/par-1^RNAi.UAS.cZa is a suppressor of wing hair phenotype of Scer\GAL4^en-e16E, ft^UAS.cMa

(Harumoto et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The planar polarity defects seen in the wing in animals expressing ft^Scer\UAS.cMa under the control of Scer\GAL4^en-e16E is significantly suppressed by co-expression of par-1^{dsRNA.Scer\UAS.cZa}.

(Harumoto et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

par-1^RNAi.UAS.cZa

par-1^{dsRNA.Scer\UAS.cZa}

par-1^{dsRNA.UAS.cZa}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close