FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Mdr65G1190D.UAS.GFP
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General Information
Symbol
Dmel\Mdr65G1190D.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0247677
Feature type
allele
Associated gene
Dmel\Mdr65
Associated Insertion(s)
Carried in Construct
P{UAS-Mdr65-GFP.G1190D}
Key Links
Transgenic product class
missense_variant
(Mayer et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Mayer et al., 2009)
Mutagen
in vitro construct
(Mayer et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Mdr65-GFP.G1190D}
Cytology
Description

Amino acid replacement: G1190D.

(Mayer et al., 2009)

UAS regulatory sequences drive expression of mutated Mdr65 coding sequences (from RE14657) tagged at the C terminal with GFP.

(Mayer et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mdr65
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Mdr65G1190D.Scer\UAS.T:Avic\GFP in the humoral interface (at the blood-brain barrier) under the control of Scer\GAL4SPG significantly decreases brain-specific Rhodamine B content, implicating a role for Mdr65 in blood-brain barrier transport.

      (Mayer et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Mdr65G1190D.UAS.GFP/Scer\GAL4moody.SPG rescues Mdr65Ex8

      (Mayer et al., 2009)
      Comments

      Expression of Mdr65G1190D.Scer\UAS.T:Avic\GFP at the humoral interface under the control of Scer\GAL4SPG is sufficient to restore efflux transport of small-molecule fluors, indicating that a role of Mdr65 in CNS protection is localized to the SPG.

      (Mayer et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mdr65G1190D.Scer\UAS.T:Avic\GFP
      Mdr65G1190D.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)