FlyBase Allele Report: Dmel\p53[UASp.cBa]

General Information

Symbol

Dmel\p53^UASp.cBa

Species

D. melanogaster

Name

FlyBase ID

FBal0248731

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{UASp-p53.B}

Key Links

Transgenic product class

wild_type

(Bakhrat et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bakhrat et al., 2010)

Mutagen

in vitro construct

(Bakhrat et al., 2010)

Progenitor genotype

Carried in construct

P{UASp-p53.B}

Cytology

Description

UASp regulatory sequences drive expression of the entire p53 ORF.

(Bakhrat et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number, with Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

decreased cell number | oogenesis, with Scer\GAL4^nanos.PG

(Martin et al., 2022)

increased cell death | germline clone, with Scer\GAL4^GR1

(Bakhrat et al., 2010)

increased cell death | germline clone, with Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

Phenotype Manifest In

egg chamber, with Scer\GAL4^GR1

(Bakhrat et al., 2010)

eye, with Scer\GAL4^GMR.PF

(Bakhrat et al., 2010)

female germline cell | decreased number, with Scer\GAL4^nanos.PG

(Martin et al., 2022)

female germline cyst, with Scer\GAL4^nanos.PG

(Martin et al., 2022)

germarium, with Scer\GAL4^nanos.PG

(Martin et al., 2022)

germline cell, with Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Bakhrat et al., 2010)

ommatidium, with Scer\GAL4^GMR.PF

(Bakhrat et al., 2010)

ovary, with Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

Detailed Description

Statement

Reference

The expression of p53^UASp.cBa under the control of Scer\GAL4^nos.PG leads to most germaria showing a complete loss of germline cells. Unlike controls, this expression leads to ovaries with 'stem cysts' proximal to the self-renewal niche, where cells are interlinked by fusome-like structures, and lacking spectrosome-containing cells;

(Martin et al., 2022)

Overexpression of p53^{Scer\UAS.P\T.cBa} in the eye using Scer\GAL4^GMR.PF results in a reduced eye size with partial fusion of the ommatidia and some remaining bristles due to apoptosis.

Females overexpressing p53^{Scer\UAS.P\T.cBa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, which drives expression during ovarian stem cell development and also during oogenesis, are agametic with rudimentary ovaries probably due to loss of ovarian stem cells.

Overexpression of p53^{Scer\UAS.P\T.cBa} in the follicle somatic cells using Scer\GAL4^GR1 induces germline cell death and leads to egg chamber death.

Expression of p53^{Scer\UAS.P\T.cBa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} significantly lowers the number of primordial germ cells in larval gonads. This appears to be through a reduction in primordial germ cell proliferation, rather than an increase in cell death (both apoptosis and autophagy).

(Bakhrat et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, p53^UASp.cBa has increased cell death | germline clone phenotype, non-suppressible by Diap1^UASp.cPa, Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

Scer\GAL4^{VP16.nanos.UTR}, p53^UASp.cBa has increased cell death | germline clone phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

Scer\GAL4^{VP16.nanos.UTR}, p53^UASp.cBa has increased cell death | germline clone phenotype, non-suppressible by NPV(Sl)\P49^UASp.cBa, Scer\GAL4^{VP16.nanos.UTR}

(Bakhrat et al., 2010)

NOT Suppressor of

Statement

Reference

p53^UASp.cBa/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of increased cell death phenotype of Scer\GAL4^{VP16.mat.αTub67C}, lok^UASp.Tag:HA

(Bakhrat et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UASp.cBa has eye phenotype, enhanceable by lok^UASp.Tag:HA/Scer\GAL4^GMR.PF

(Bakhrat et al., 2010)

NOT Suppressor of

Statement

Reference

p53^UASp.cBa/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of egg chamber | oogenesis stage S8 phenotype of Scer\GAL4^{VP16.mat.αTub67C}, lok^UASp.Tag:HA

(Bakhrat et al., 2010)

p53^UASp.cBa/Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of egg chamber | oogenesis stage S9 phenotype of Scer\GAL4^{VP16.mat.αTub67C}, lok^UASp.Tag:HA

(Bakhrat et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of lok^{Scer\UAS.P\T.T:Ivir\HA1} with p53^{Scer\UAS.P\T.cBa} under the control of Scer\GAL4^GMR.PF greatly enhances the p53^{Scer\UAS.P\T.cBa} phenotype with almost complete loss of the eye.

Co-expression of lok^{Scer\UAS.P\T.T:Ivir\HA1} with p53^{Scer\UAS.P\T.cBa} during mid-oogenesis under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not block lok^{Scer\UAS.P\T.T:Ivir\HA1}-dependent cell death. The level of cell death of egg chambers is similar as when lok^{Scer\UAS.P\T.T:Ivir\HA1} is overexpressed alone.

Overexpression of th^{Scer\UAS.P\T.cPa} with p53^{Scer\UAS.P\T.cBa}, during oogenesis under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the p53^{Scer\UAS.P\T.cBa}-dependent stem cell loss.

(Bakhrat et al., 2010)

Xenogenetic Interactions

Statement

Reference

Overexpression of BacA\p35^Scer\UAS.cHa with p53^{Scer\UAS.P\T.cBa}, during oogenesis under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the p53^{Scer\UAS.P\T.cBa}-dependent stem cell loss.

Overexpression of NPV(Sl)\P49^{Scer\UAS.P\T.cBa} with p53^{Scer\UAS.P\T.cBa}, during oogenesis under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the p53^{Scer\UAS.P\T.cBa}-dependent stem cell loss.

(Bakhrat et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p53^{Scer\UAS.P\T.cBa}

p53^UASp.cBa

Name Synonyms

Secondary FlyBase IDs

References (2)

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