FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4Gr64e.8
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General Information
Symbol
Scer\GAL4Gr64e.8
Species
S. cerevisiae
Name
FlyBase ID
FBal0249508
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Gr64e-GAL4.8}
Also Known As
Gr64e-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Weiss et al., 2011)
Progenitor genotype
Carried in construct
P{Gr64e-GAL4.8}
Cytology
Description

A 8kb Gr64e promoter fragment is fused upstream of sequence encoding a GAL4 driver.

(Weiss et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Gr64e
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      sugar-sensing neuron of taste bristle | subset of prothoracic tarsal segment 2
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of prothoracic tarsal segment 3
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of prothoracic tarsal segment 5
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of prothoracic tarsal segment 4
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of mesothoracic tarsal segment 5
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of mesothoracic tarsal segment 4
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of metathoracic tarsal segment 5
      (Ling et al., 2014)
      sugar-sensing neuron of taste bristle | subset of metathoracic tarsal segment 4
      (Ling et al., 2014)
      gustatory receptor neuron of adult labellum sensillum | subset
      (Weiss et al., 2011)
      sugar-sensing neuron of taste bristle of adult labellum sensillum
      (Weiss et al., 2011)
      gustatory receptor neuron of labellar taste peg | subset
      (Wisotsky et al., 2011)
      gustatory receptor neuron of labellar taste bristle | subset
      (Wisotsky et al., 2011)
      labral sense organ neuron | subset
      (Wisotsky et al., 2011)
      gustatory receptor neuron of leg taste bristle | subset
      (Wisotsky et al., 2011)
      adult gnathal ganglion | restricted
      (Wisotsky et al., 2011)
      adult labial nerve
      (Wisotsky et al., 2011)
      adult pharyngeal nerve
      (Wisotsky et al., 2011)
      adult cervical connective
      (Wisotsky et al., 2011)
      L-type sensillum L1
      (Weiss et al., 2011)
      L-type sensillum L2
      (Weiss et al., 2011)
      L-type sensillum L4
      (Weiss et al., 2011)
      L-type sensillum L6
      (Weiss et al., 2011)
      L-type sensillum L8
      (Weiss et al., 2011)
      L-type sensillum L9
      (Weiss et al., 2011)
      L-type sensillum L3
      (Weiss et al., 2011)
      L-type sensillum L5
      (Weiss et al., 2011)
      L-type sensillum L7
      (Weiss et al., 2011)
      bitter-sensitive labellar taste bristle
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-a
      (Weiss et al., 2011)
      intermediate labellar taste bristle
      (Weiss et al., 2011)
      short labellar taste bristle S-b
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-b
      (Weiss et al., 2011)
      prothoracic leg taste bristle ascending tarsal chemosensory neuron
      (Thoma et al., 2016)
      prothoracic leg taste bristle segmental tarsal chemosensory neuron
      (Thoma et al., 2016)
      adult abdominal multidendritic neuron | subset
      (Park and Kwon, 2011)
      short labellar taste bristle S-a
      (Weiss et al., 2011)
      Additional Information
      Statement
      Reference

      ScerGAL4Gr64e.8 drives expression in about 12 sugar-sensing chemosensory neurons in the prothoracic leg. A subset of the leg neurons project to the ventral nerve cord then ascend through the cervical connective to the posterior gnathal gangion.

      (Thoma et al., 2016)

      ScerGAL4Gr64e.8 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in multidendritic neurons with ladder-like processes along the dorsal abdominal wall.

      (Park and Kwon, 2011)

      ScerGAL4Gr64e.8 is expressed in the sugar-responsive neuron of almost all of the short, intermediate and long taste sensilla of the labellum.

      (Weiss et al., 2011)

      ScerGAL4Gr64e.8 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

      (Weiss et al., 2011)

      ScerGAL4Gr64e.8 drives expression in the gustatory receptor neurons of a subset of the labellar taste pegs and bristles, the labral sense organ and taste bristles in the leg. The gustatory receptor neurons projections in the brain are also labelled with ScerGAL4Gr64e.8. These projections label the labial and pharyngeal nerves, and cervical connective and their entry point in the subesophageal ganglion. The projections of the gustatory receptor neurons labelled with ScerGAL4Gr64e.8 are located close to the projections of sugar-sensitive neurons.

      (Wisotsky et al., 2011)
       
      Marker for
      Reflects expression of
      Gr64e
      (Thoma et al., 2016, Ling et al., 2014, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011, Wisotsky et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr64e.8 cells in adults significantly reduces preference for sucrose, compared to controls.

      (Thoma et al., 2016)

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with orange light) of Scer\GAL4Gr64e.8 neurons in the absence of food prior to testing in adults does not affect food intake, compared to controls.

      (Zhan et al., 2016)

      Inhibition (using Ork1Δ-C.UAS.EGFP) of Scer\GAL4Gr64e.8 cells in adults given a choice between sucrose solution and water does not affect water or sucrose consumption, compared to controls.

      (Sun et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Gr64e.8
      Name Synonyms
      Secondary FlyBase IDs
        References (15)